Identification of the functional subunit of a dimeric transcription activator protein by use of oriented heterodimers

Yuhong Zhou; Steve Busby; Richard H. Ebright

doi:10.1016/0092-8674(93)90236-J

Identification of the functional subunit of a dimeric transcription activator protein by use of oriented heterodimers

Yuhong Zhou, Steve Busby, Richard H. Ebright

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Abstract

We have constructed heterodimers consisting of two subunits: one CAP subunit that has a nonfunctional activating region but wild-type DNA binding specificity, and one CAP subunit that has a functional activating region but non-wild-type DNA binding specificity. We have oriented the heterodimers on lac promoter DNA by use of promoter derivatives that have DNA sites for CAP consisting of one wild-type half site and one non-wild-type half site, and we have analyzed the abilities of the oriented heterodimers to activate transcription. Our results indicate that transcription activation requires the activating region of only one subunit of CAP: the promoter-proximal subunit. The oriented heterodimers method of this report should be generalizable to other dimeric transcription activator proteins.

Original language	English (US)
Pages (from-to)	375-379
Number of pages	5
Journal	Cell
Volume	73
Issue number	2
DOIs	https://doi.org/10.1016/0092-8674(93)90236-J
State	Published - Apr 23 1993

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

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10.1016/0092-8674(93)90236-J

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@article{72d3e15a779143deb2d1a43671c11a8d,

title = "Identification of the functional subunit of a dimeric transcription activator protein by use of oriented heterodimers",

abstract = "We have constructed heterodimers consisting of two subunits: one CAP subunit that has a nonfunctional activating region but wild-type DNA binding specificity, and one CAP subunit that has a functional activating region but non-wild-type DNA binding specificity. We have oriented the heterodimers on lac promoter DNA by use of promoter derivatives that have DNA sites for CAP consisting of one wild-type half site and one non-wild-type half site, and we have analyzed the abilities of the oriented heterodimers to activate transcription. Our results indicate that transcription activation requires the activating region of only one subunit of CAP: the promoter-proximal subunit. The oriented heterodimers method of this report should be generalizable to other dimeric transcription activator proteins.",

author = "Yuhong Zhou and Steve Busby and Ebright, {Richard H.}",

note = "Funding Information: We thank Drs. A. Kolb and H. But for plasmids pBR-203-lacPL29 and pBR-203-lacPL8. This work was supported by National Institutes of Health grant GM41376 to Ft. H. E., an EPA Cephalosporin Research Fellowship to S. B., and a Busch Predoctoral Fellowship to Y. 2.",

year = "1993",

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doi = "10.1016/0092-8674(93)90236-J",

language = "English (US)",

volume = "73",

pages = "375--379",

journal = "Cell",

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publisher = "Cell Press",

number = "2",

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T1 - Identification of the functional subunit of a dimeric transcription activator protein by use of oriented heterodimers

AU - Zhou, Yuhong

AU - Busby, Steve

AU - Ebright, Richard H.

N1 - Funding Information: We thank Drs. A. Kolb and H. But for plasmids pBR-203-lacPL29 and pBR-203-lacPL8. This work was supported by National Institutes of Health grant GM41376 to Ft. H. E., an EPA Cephalosporin Research Fellowship to S. B., and a Busch Predoctoral Fellowship to Y. 2.

PY - 1993/4/23

Y1 - 1993/4/23

N2 - We have constructed heterodimers consisting of two subunits: one CAP subunit that has a nonfunctional activating region but wild-type DNA binding specificity, and one CAP subunit that has a functional activating region but non-wild-type DNA binding specificity. We have oriented the heterodimers on lac promoter DNA by use of promoter derivatives that have DNA sites for CAP consisting of one wild-type half site and one non-wild-type half site, and we have analyzed the abilities of the oriented heterodimers to activate transcription. Our results indicate that transcription activation requires the activating region of only one subunit of CAP: the promoter-proximal subunit. The oriented heterodimers method of this report should be generalizable to other dimeric transcription activator proteins.

AB - We have constructed heterodimers consisting of two subunits: one CAP subunit that has a nonfunctional activating region but wild-type DNA binding specificity, and one CAP subunit that has a functional activating region but non-wild-type DNA binding specificity. We have oriented the heterodimers on lac promoter DNA by use of promoter derivatives that have DNA sites for CAP consisting of one wild-type half site and one non-wild-type half site, and we have analyzed the abilities of the oriented heterodimers to activate transcription. Our results indicate that transcription activation requires the activating region of only one subunit of CAP: the promoter-proximal subunit. The oriented heterodimers method of this report should be generalizable to other dimeric transcription activator proteins.

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DO - 10.1016/0092-8674(93)90236-J

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JO - Cell

JF - Cell

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Identification of the functional subunit of a dimeric transcription activator protein by use of oriented heterodimers

Abstract

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