Identification of the functional subunit of a dimeric transcription activator protein by use of oriented heterodimers

Yuhong Zhou, Steve Busby, Richard H. Ebright

Research output: Contribution to journalArticlepeer-review

70 Scopus citations

Abstract

We have constructed heterodimers consisting of two subunits: one CAP subunit that has a nonfunctional activating region but wild-type DNA binding specificity, and one CAP subunit that has a functional activating region but non-wild-type DNA binding specificity. We have oriented the heterodimers on lac promoter DNA by use of promoter derivatives that have DNA sites for CAP consisting of one wild-type half site and one non-wild-type half site, and we have analyzed the abilities of the oriented heterodimers to activate transcription. Our results indicate that transcription activation requires the activating region of only one subunit of CAP: the promoter-proximal subunit. The oriented heterodimers method of this report should be generalizable to other dimeric transcription activator proteins.

Original languageEnglish (US)
Pages (from-to)375-379
Number of pages5
JournalCell
Volume73
Issue number2
DOIs
StatePublished - Apr 23 1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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