Identification of the Myxococcus xanthus 59-kDa membrane-associated GTP-binding protein as a proton-translocating ATPase

José Muñoz-Dorado, Sumiko Inouye, Masayori Inouye

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Five GTP-binding proteins have been detected in Myxococcus xanthus by photoaffinity cross-linking with azido-GTP [Muñoz-Dorado et al., J. Biol. Chem. 265 (1990a) 2702-2706]. One of them, the 59-kDa membrane-associated GTP-binding protein, has been purified. The N-terminal sequence of a 10-kDa fragment from the protease V8 digestion of the purified protein has been determined and degenerate oligodeoxyribonucleotides based on that sequence have been used to isolate and clone the gene that encodes the GTP-binding protein. The gene was sequenced and further analysis of the sequence revealed that the protein encoded by this gene shows very high homology with the a subunit of protontranslocating ATPases.

Original languageEnglish (US)
Pages (from-to)133-137
Number of pages5
JournalGene
Volume138
Issue number1-2
DOIs
StatePublished - Jan 28 1994

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • Myxobacteria
  • alpha subunit of ATPase
  • azido-GTP
  • membrane protein

Fingerprint

Dive into the research topics of 'Identification of the Myxococcus xanthus 59-kDa membrane-associated GTP-binding protein as a proton-translocating ATPase'. Together they form a unique fingerprint.

Cite this