Identification of the packaging regions within the genomic RNA segments of bacteriophage Φ6

Paul Gottlieb, Xueying Qiao, Jeffrey Strassman, Mikko Frilander, Leonard Mindich

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

Bacteriophage Φ6 has a genome of three segments of double-stranded RNA enclosed in a procapsid composed of four different proteins. The preformed procapsid is capable of packaging plus-strand transcripts of the genomic segments in an in vitro reaction. The packaging-specific sequences on the RNA molecules are located near the 5' ends. In this study we show that the packaging sequences are different for each of the three segments and that they are of about 250 nucleotides in length. Although these sequences are consistent with some secondary structure, there is no clear structural similarity between the packaging regions of the three segments.

Original languageEnglish (US)
Article number71160
Pages (from-to)42-47
Number of pages6
JournalVirology
Volume200
Issue number1
DOIs
StatePublished - Apr 1994

All Science Journal Classification (ASJC) codes

  • Virology

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