Identification of thyroid regulatory elements in the Na-K-ATPase α3 gene promoter

Manisha Bajpai; Swapan K. Mandal; Sukanya Chaudhury

doi:10.1023/A:1011986418897

Identification of thyroid regulatory elements in the Na-K-ATPase α₃ gene promoter

Manisha Bajpai, Swapan K. Mandal, Sukanya Chaudhury

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A - 1027 bp to + 108 bp region of Na-K-ATPase α₃ gene promoter has been searched for the presence of thyroid response elements (TRE). Computer analysis of this sequence using a consensus TRE sequence revealed the presence of four putative TRE rich regions referred to as regions I (-636 to -457 bp), II (-218 to -106 bp), III (-106 to -6 bp) and IV (-6 to +108 bp). Cotransfection of the luciferase linked full length construct as well as constructs progressively devoid of the TRE rich regions in Cos1 cells revealed that regions I and III are positively regulated by T₃ whereas there are some sequences in region II which can suppress the positive regulatory effect of region III but not of region I, TRE IV seems to have no functional role. EMSA of the three functional TRE rich regions (I, II and III) showed strong and specific interaction with thyroid hormone receptor (TR) cloned and expressed in baculovirus. The overall results suggest the regulation of Na-K-ATPase α₃ gene by T₃ is complex involving several thyroidal regulatory elements.

Original language	English (US)
Pages (from-to)	1-7
Number of pages	7
Journal	Molecular Biology Reports
Volume	28
Issue number	1
DOIs	https://doi.org/10.1023/A:1011986418897
State	Published - 2001
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

Keywords

Na-K-ATPase α gene
Thyroid response elements

Access to Document

10.1023/A:1011986418897

Cite this

@article{68eeaac4f2e7407dbdd01f434a193fba,

title = "Identification of thyroid regulatory elements in the Na-K-ATPase α3 gene promoter",

abstract = "A - 1027 bp to + 108 bp region of Na-K-ATPase α3 gene promoter has been searched for the presence of thyroid response elements (TRE). Computer analysis of this sequence using a consensus TRE sequence revealed the presence of four putative TRE rich regions referred to as regions I (-636 to -457 bp), II (-218 to -106 bp), III (-106 to -6 bp) and IV (-6 to +108 bp). Cotransfection of the luciferase linked full length construct as well as constructs progressively devoid of the TRE rich regions in Cos1 cells revealed that regions I and III are positively regulated by T3 whereas there are some sequences in region II which can suppress the positive regulatory effect of region III but not of region I, TRE IV seems to have no functional role. EMSA of the three functional TRE rich regions (I, II and III) showed strong and specific interaction with thyroid hormone receptor (TR) cloned and expressed in baculovirus. The overall results suggest the regulation of Na-K-ATPase α3 gene by T3 is complex involving several thyroidal regulatory elements.",

keywords = "Na-K-ATPase α gene, Thyroid response elements",

author = "Manisha Bajpai and Mandal, {Swapan K.} and Sukanya Chaudhury",

note = "Funding Information: This work was supported by a grant from the Department of Science and Technology, Govt. of India. We are grateful to Dr J.B. Lingrel for his kind gift of the plasmid pRα3 -1027 and to Dr P.K. Sarkar for his guidance in carrying out the work and for the review of this manuscript.",

year = "2001",

doi = "10.1023/A:1011986418897",

language = "English (US)",

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TY - JOUR

T1 - Identification of thyroid regulatory elements in the Na-K-ATPase α3 gene promoter

AU - Bajpai, Manisha

AU - Mandal, Swapan K.

AU - Chaudhury, Sukanya

N1 - Funding Information: This work was supported by a grant from the Department of Science and Technology, Govt. of India. We are grateful to Dr J.B. Lingrel for his kind gift of the plasmid pRα3 -1027 and to Dr P.K. Sarkar for his guidance in carrying out the work and for the review of this manuscript.

PY - 2001

Y1 - 2001

N2 - A - 1027 bp to + 108 bp region of Na-K-ATPase α3 gene promoter has been searched for the presence of thyroid response elements (TRE). Computer analysis of this sequence using a consensus TRE sequence revealed the presence of four putative TRE rich regions referred to as regions I (-636 to -457 bp), II (-218 to -106 bp), III (-106 to -6 bp) and IV (-6 to +108 bp). Cotransfection of the luciferase linked full length construct as well as constructs progressively devoid of the TRE rich regions in Cos1 cells revealed that regions I and III are positively regulated by T3 whereas there are some sequences in region II which can suppress the positive regulatory effect of region III but not of region I, TRE IV seems to have no functional role. EMSA of the three functional TRE rich regions (I, II and III) showed strong and specific interaction with thyroid hormone receptor (TR) cloned and expressed in baculovirus. The overall results suggest the regulation of Na-K-ATPase α3 gene by T3 is complex involving several thyroidal regulatory elements.

AB - A - 1027 bp to + 108 bp region of Na-K-ATPase α3 gene promoter has been searched for the presence of thyroid response elements (TRE). Computer analysis of this sequence using a consensus TRE sequence revealed the presence of four putative TRE rich regions referred to as regions I (-636 to -457 bp), II (-218 to -106 bp), III (-106 to -6 bp) and IV (-6 to +108 bp). Cotransfection of the luciferase linked full length construct as well as constructs progressively devoid of the TRE rich regions in Cos1 cells revealed that regions I and III are positively regulated by T3 whereas there are some sequences in region II which can suppress the positive regulatory effect of region III but not of region I, TRE IV seems to have no functional role. EMSA of the three functional TRE rich regions (I, II and III) showed strong and specific interaction with thyroid hormone receptor (TR) cloned and expressed in baculovirus. The overall results suggest the regulation of Na-K-ATPase α3 gene by T3 is complex involving several thyroidal regulatory elements.

KW - Na-K-ATPase α gene

KW - Thyroid response elements

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