Identification of XLerk, an Eph family ligand regulated during mesoderm induction and neurogenesis in Xenopus laevis

Teri L. Jones, Irina Karavanova, Lisa Chong, Renping Zhou, Ira O. Daar

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have isolated and characterized the first Xenopus transmembrane Eph ligand, XLerk (Xenopus Ligand for Eph Receptor Tyrosine Kinases). While this ligand has 72% identity with the closest mammalian family member, Lerk-2, it is the cytoplasmic domain of this molecule that is the most conserved domain with 95% identity. XLerk exists as a maternally expressed mRNA, however, expression of transcripts and protein increase during gastrulation and again in the late swimming tadpole stage. In the adult, XLerk is expressed at low levels in most adult tissues with increased levels observed in the kidney, oocytes, ovary and testis. While low levels of XLerk expression are observed in the adult brain, in situ hybridization analysis demonstrates prominent expression in the developing olfactory system, retina, hindbrain, cranial ganglia, and somites. Furthermore, we have shown that XLerk transcripts are significantly elevated during mesoderm induction caused by activin and FGF, but not during noggin-induced neuralization. These results suggest a role for XLerk in the developing mesenchymal and nervous tissue.

Original languageEnglish (US)
Pages (from-to)2159-2166
Number of pages8
JournalOncogene
Volume14
Issue number18
DOIs
StatePublished - Jan 1 1997

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Eph Family Receptors
Neurogenesis
Xenopus laevis
Mesoderm
Xenopus
Ligands
Nerve Tissue
Activins
Somites
Gastrulation
Rhombencephalon
Ganglia
Oocytes
In Situ Hybridization
Larva
Retina
Testis
Ovary
Kidney
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Jones, Teri L. ; Karavanova, Irina ; Chong, Lisa ; Zhou, Renping ; Daar, Ira O. / Identification of XLerk, an Eph family ligand regulated during mesoderm induction and neurogenesis in Xenopus laevis. In: Oncogene. 1997 ; Vol. 14, No. 18. pp. 2159-2166.
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Identification of XLerk, an Eph family ligand regulated during mesoderm induction and neurogenesis in Xenopus laevis. / Jones, Teri L.; Karavanova, Irina; Chong, Lisa; Zhou, Renping; Daar, Ira O.

In: Oncogene, Vol. 14, No. 18, 01.01.1997, p. 2159-2166.

Research output: Contribution to journalArticle

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