Identifying citrullination sites by mass spectrometry

Jennifer E. Grant, Hong Li

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The citrullination of arginine is one of the smallest posttranslational modifications made to proteins, by mass. Mass spectrometric methods of analysis offer the potential to not only identify sites of protein citrullination but in some instances to quantify their occurrence in diseased tissue, relative to healthy tissues. This is an emerging, if challenging, area where method and instrument selections can influence not only data quality but also throughput. Citrullination, or deimination of arginine, results in the addition of only 0.9848 Da to a peptide. Recent improvements in the accuracy and resolution of mass spectrometers, as well as the advancement of protein chemistry technology that selectively targets citrulline moieties, has allowed greater success in identifying citrullinated arginine within proteins and peptides. This chapter provides an overview of recent, successful strategies used to identify citrullination sites using modern proteomic methods that involve mass spectrometric analysis. Applications to the study of multiple sclerosis and arthritis are highlighted.

Original languageEnglish (US)
Title of host publicationProtein Deimination in Human Health and Disease
PublisherSpringer New York
Pages347-365
Number of pages19
ISBN (Electronic)9781461483175
ISBN (Print)1461483166, 9781461483168
DOIs
StatePublished - Jan 1 2014

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Neuroscience(all)

Keywords

  • Arginine deimination
  • Citrullination
  • Mass spectrometry
  • Posttranslational modification

Fingerprint Dive into the research topics of 'Identifying citrullination sites by mass spectrometry'. Together they form a unique fingerprint.

  • Cite this

    Grant, J. E., & Li, H. (2014). Identifying citrullination sites by mass spectrometry. In Protein Deimination in Human Health and Disease (pp. 347-365). Springer New York. https://doi.org/10.1007/978-1-4614-8317-5_19