Identifying the assembly pathway of cyanophage inside the marine bacterium using electron cryo-tomography

Wei Dai, Michael F. Schmid, Jonathan A. King, Wah Chiu

Research output: Contribution to journalReview articlepeer-review

5 Scopus citations

Abstract

Advances in electron cryo-tomography open up a new avenue to visualize the 3-D internal structure of a single bacterium before and after its infection by bacteri-ophages in its native environment, without using chemical fixatives, fluorescent dyes or negative stains. Such direct observation reveals the presence of assembly intermediates of the bacteriophage and thus allows us to map out the maturation pathway of the bacteriophage inside its host.

Original languageEnglish (US)
Pages (from-to)45-47
Number of pages3
JournalMicrobial Cell
Volume1
Issue number1
DOIs
StatePublished - Jan 2014
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Microbiology (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

Keywords

  • 3-D structures
  • Electron cryo-tomography
  • Marine cyanobacterium
  • Phage assembly intermediates
  • Transient biological processes
  • Zernike phase optics

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