IGF1 gene transfer into skeletal muscle using recombinant adeno-associated virus in a rat model of liver cirrhosis

M. Zaratiegui, I. Castilla-Cortázar, M. García, J. Quiroga, J. Prieto, F. J. Novo

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Systemic administration of recombinant IGF1 at low levels has been shown to improve hepatic function, nutritional status and testicular atrophy in rats with CCl4-induced cirrhosis. We have developed a recombinant adeno-associated (rAAV) viral vector containing the cDNA for rat IGF1 and confirmed the expression of IGF1 after intramuscular injection of this vector in a rat model of liver cirrhosis. Although weight of injected muscles was significantly increased in rats with mild cirrhosis, this was not the case in rats with advanced, de-compensated cirrhosis. Furthermore, we found no significant amelioration of liver damage in treated rats at any stage of liver cirrhosis. Our results suggest that IGF1 gene transfer into muscle results in a local effect, at least at the vector dose employed here.

Original languageEnglish (US)
Pages (from-to)169-176
Number of pages8
JournalJournal of Physiology and Biochemistry
Volume58
Issue number3
DOIs
StatePublished - Sep 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology

Keywords

  • Adeno-associated virus
  • Cirrhosis
  • Insulin-like factor 1

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