IL-13 and IL-4 promote TARC release in human airway smooth muscle cells: Role of IL-4 receptor genotype

Débora S. Faffe, Timothy Whitehead, Paul E. Moore, Simonetta Baraldo, Lesley Flynt, Kerri Bourgeois, Reynold Panettieri, Stephanie A. Shore

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The chemokine thymus- and activation-regulated chemokine (TARC) induces selective migration of Th2, but not Th1, lymphocytes and is upregulated in the airways of asthmatic patients. The purpose of this study was to determine whether human airway smooth muscle (HASM) cells produce TARC. Neither IL-4, IL-13, IL-1β, IFN-γ, nor TNF-α alone stimulated TARC release into the supernatant of cultured HASM cells. However, both IL-4 and IL-13 increased TARC protein and mRNA expression when administered in combination with TNF-α but not IL-1β or IFN-γ. Macrophage-derived chemokine was not expressed under any of these conditions. TARC release induced by TNF-α + IL-13 or TNF-α + IL-4 was inhibited by the β-agonist isoproterenol and by other agents that activate protein kinase A, but not by dexamethasone. To determine whether polymorphisms of the IL-4Rα have an impact on the ability of IL-13 or IL-4 to induce TARC release, HASM cells from multiple donors were genotyped for the Ile50Val, Ser478Pro, and Gln551Arg polymorphisms of the IL-4Rα. Our data indicate that cells expressing the Val50/Pro478/Arg551 haplotype had significantly greater IL-13- or IL-4-induced TARC release than cells with other IL-4Rα genotypes. These data indicate that Th2 cytokines enhance TARC expression in HASM cells in an IL-4Rα genotype-dependent fashion and suggest that airway smooth muscle cells participate in a positive feedback loop that promotes the recruitment of Th2 cells into asthmatic airways.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume285
Issue number4 29-4
StatePublished - Oct 1 2003
Externally publishedYes

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Chemokine CCL17
Interleukin-4 Receptors
Interleukin-13
Interleukin-4
Smooth Muscle Myocytes
Genotype
Interleukin-1
Chemokine CCL22
Th2 Cells
Cyclic AMP-Dependent Protein Kinases
Isoproterenol
Chemokines
Haplotypes
Dexamethasone
Tissue Donors
Lymphocytes
Cytokines
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology

Cite this

Faffe, D. S., Whitehead, T., Moore, P. E., Baraldo, S., Flynt, L., Bourgeois, K., ... Shore, S. A. (2003). IL-13 and IL-4 promote TARC release in human airway smooth muscle cells: Role of IL-4 receptor genotype. American Journal of Physiology - Lung Cellular and Molecular Physiology, 285(4 29-4).
Faffe, Débora S. ; Whitehead, Timothy ; Moore, Paul E. ; Baraldo, Simonetta ; Flynt, Lesley ; Bourgeois, Kerri ; Panettieri, Reynold ; Shore, Stephanie A. / IL-13 and IL-4 promote TARC release in human airway smooth muscle cells : Role of IL-4 receptor genotype. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 2003 ; Vol. 285, No. 4 29-4.
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IL-13 and IL-4 promote TARC release in human airway smooth muscle cells : Role of IL-4 receptor genotype. / Faffe, Débora S.; Whitehead, Timothy; Moore, Paul E.; Baraldo, Simonetta; Flynt, Lesley; Bourgeois, Kerri; Panettieri, Reynold; Shore, Stephanie A.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 285, No. 4 29-4, 01.10.2003.

Research output: Contribution to journalArticle

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T1 - IL-13 and IL-4 promote TARC release in human airway smooth muscle cells

T2 - Role of IL-4 receptor genotype

AU - Faffe, Débora S.

AU - Whitehead, Timothy

AU - Moore, Paul E.

AU - Baraldo, Simonetta

AU - Flynt, Lesley

AU - Bourgeois, Kerri

AU - Panettieri, Reynold

AU - Shore, Stephanie A.

PY - 2003/10/1

Y1 - 2003/10/1

N2 - The chemokine thymus- and activation-regulated chemokine (TARC) induces selective migration of Th2, but not Th1, lymphocytes and is upregulated in the airways of asthmatic patients. The purpose of this study was to determine whether human airway smooth muscle (HASM) cells produce TARC. Neither IL-4, IL-13, IL-1β, IFN-γ, nor TNF-α alone stimulated TARC release into the supernatant of cultured HASM cells. However, both IL-4 and IL-13 increased TARC protein and mRNA expression when administered in combination with TNF-α but not IL-1β or IFN-γ. Macrophage-derived chemokine was not expressed under any of these conditions. TARC release induced by TNF-α + IL-13 or TNF-α + IL-4 was inhibited by the β-agonist isoproterenol and by other agents that activate protein kinase A, but not by dexamethasone. To determine whether polymorphisms of the IL-4Rα have an impact on the ability of IL-13 or IL-4 to induce TARC release, HASM cells from multiple donors were genotyped for the Ile50Val, Ser478Pro, and Gln551Arg polymorphisms of the IL-4Rα. Our data indicate that cells expressing the Val50/Pro478/Arg551 haplotype had significantly greater IL-13- or IL-4-induced TARC release than cells with other IL-4Rα genotypes. These data indicate that Th2 cytokines enhance TARC expression in HASM cells in an IL-4Rα genotype-dependent fashion and suggest that airway smooth muscle cells participate in a positive feedback loop that promotes the recruitment of Th2 cells into asthmatic airways.

AB - The chemokine thymus- and activation-regulated chemokine (TARC) induces selective migration of Th2, but not Th1, lymphocytes and is upregulated in the airways of asthmatic patients. The purpose of this study was to determine whether human airway smooth muscle (HASM) cells produce TARC. Neither IL-4, IL-13, IL-1β, IFN-γ, nor TNF-α alone stimulated TARC release into the supernatant of cultured HASM cells. However, both IL-4 and IL-13 increased TARC protein and mRNA expression when administered in combination with TNF-α but not IL-1β or IFN-γ. Macrophage-derived chemokine was not expressed under any of these conditions. TARC release induced by TNF-α + IL-13 or TNF-α + IL-4 was inhibited by the β-agonist isoproterenol and by other agents that activate protein kinase A, but not by dexamethasone. To determine whether polymorphisms of the IL-4Rα have an impact on the ability of IL-13 or IL-4 to induce TARC release, HASM cells from multiple donors were genotyped for the Ile50Val, Ser478Pro, and Gln551Arg polymorphisms of the IL-4Rα. Our data indicate that cells expressing the Val50/Pro478/Arg551 haplotype had significantly greater IL-13- or IL-4-induced TARC release than cells with other IL-4Rα genotypes. These data indicate that Th2 cytokines enhance TARC expression in HASM cells in an IL-4Rα genotype-dependent fashion and suggest that airway smooth muscle cells participate in a positive feedback loop that promotes the recruitment of Th2 cells into asthmatic airways.

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