Immune responses to mouse neuroblastoma C1300. I. preliminary observations on cell-mediated immune responses

Mildred E. Phillips, Patricia M. Cipuzak, Dennis Della Penta, Melitta Schachner

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

In vitro cell-mediated cytotoxicity (CMC) for [3H]proline-labeled target cells was demonstrated with the use o unfractionated populations of regional lymph node, spleen, anc peritoneal cells (RLNC’s, SPC’s, PC’s, respectively) from C57BL/(and strain A mice. Syngeneic and allogeneic hosts were immu nized sc or ip with C1300 tumor or syngeneic SPC’s. The syn geneic and allogeneic host effector lymphoid cells showec various degrees of cytotoxicity for C1300 target cells 3-9 days after one immunization with C1300, whereas the effector lym phoid cells of hosts immunized with syngeneic SPC’s generally showed less CMC for C1300 and frequently increased the growth of C1300 target cells when compared to C1300 targets plus media controls. Effector cells obtained from lymphoid organs in ths region nearest the immunization (i.e., RLNC from sc-inoculatec hosts) demonstrated significantly more CMC than did effectoi cells from more remote lymphoid organs. The PC’s and SPC’s ol C1300 ip hyperimmunized allogeneic hosts produced greatei CMC than did those of mice immunized once. This was not observed if syngeneic C1300 or SPC’s were used as hyperim munizing antigens. The CMC of nonlmmunized host effector lym' phoid cells for syngeneic labeled target cells was demonstrated.

Original languageEnglish (US)
Pages (from-to)1215-1219
Number of pages5
JournalJournal of the National Cancer Institute
Volume59
Issue number4
DOIs
StatePublished - Oct 1977
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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