Immunofluorescence and subsequent confocal microscopy of intracellular TNF in human neutrophils

Janet Rollins; Veronika Miskolci

doi:10.1007/978-1-4939-0928-5_24

Immunofluorescence and subsequent confocal microscopy of intracellular TNF in human neutrophils

Janet Rollins
, Veronika Miskolci

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Immunofluorescence is an important technique required to observe expression, localization and colocalization of proteins within the cell. Here we describe the immunofluorescence and subsequent confocal microscopy technique of tumor necrosis factor-α (TNF) in human neutrophils (polymorphonuclear leukocytes; PMN). The qualitative technique can be used to observe the expression pattern changes from resting to stimulated leukocytes. Colocalization with other cytokines, proteins, or organelles can be observed. This immunofluorescence technique can be done in 1–2 days.

Original language	English (US)
Pages (from-to)	263-270
Number of pages	8
Journal	Methods in Molecular Biology
Volume	1172
DOIs	https://doi.org/10.1007/978-1-4939-0928-5_24
State	Published - 2014
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
Genetics

Keywords

Confocal microscopy
Cytokines
Immunofl uorescence
Leukocytes
Neutrophils
TNF

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10.1007/978-1-4939-0928-5_24

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title = "Immunofluorescence and subsequent confocal microscopy of intracellular TNF in human neutrophils",

abstract = "Immunofluorescence is an important technique required to observe expression, localization and colocalization of proteins within the cell. Here we describe the immunofluorescence and subsequent confocal microscopy technique of tumor necrosis factor-α (TNF) in human neutrophils (polymorphonuclear leukocytes; PMN). The qualitative technique can be used to observe the expression pattern changes from resting to stimulated leukocytes. Colocalization with other cytokines, proteins, or organelles can be observed. This immunofluorescence technique can be done in 1–2 days.",

keywords = "Confocal microscopy, Cytokines, Immunofl uorescence, Leukocytes, Neutrophils, TNF",

author = "Janet Rollins and Veronika Miskolci",

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AU - Rollins, Janet

AU - Miskolci, Veronika

N1 - Publisher Copyright: © Springer Science+Business Media New York 2014.

PY - 2014

Y1 - 2014

N2 - Immunofluorescence is an important technique required to observe expression, localization and colocalization of proteins within the cell. Here we describe the immunofluorescence and subsequent confocal microscopy technique of tumor necrosis factor-α (TNF) in human neutrophils (polymorphonuclear leukocytes; PMN). The qualitative technique can be used to observe the expression pattern changes from resting to stimulated leukocytes. Colocalization with other cytokines, proteins, or organelles can be observed. This immunofluorescence technique can be done in 1–2 days.

AB - Immunofluorescence is an important technique required to observe expression, localization and colocalization of proteins within the cell. Here we describe the immunofluorescence and subsequent confocal microscopy technique of tumor necrosis factor-α (TNF) in human neutrophils (polymorphonuclear leukocytes; PMN). The qualitative technique can be used to observe the expression pattern changes from resting to stimulated leukocytes. Colocalization with other cytokines, proteins, or organelles can be observed. This immunofluorescence technique can be done in 1–2 days.

KW - Confocal microscopy

KW - Cytokines

KW - Immunofl uorescence

KW - Leukocytes

KW - Neutrophils

KW - TNF

UR - https://www.scopus.com/pages/publications/84922480295

UR - https://www.scopus.com/pages/publications/84922480295#tab=citedBy

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SP - 263

EP - 270

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Immunofluorescence and subsequent confocal microscopy of intracellular TNF in human neutrophils

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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