Immunohistochemical and electrochemical detection of serotonin in the nervous system of Fasciola hepatica, a parasitic flatworm

Suzanne C. Sukhdeo, Michael V.K. Sukhdeo

Research output: Contribution to journalArticle

7 Scopus citations


The head region of the trematode parasite Fasciola hepatica contains 47 ± 0.42 pmol/mg wet wt. of serotonin as measured by high-performance liquid chromatography coupled to electrochemical detection. The head region includes the cerebral ganglia, the transverse commissure and associated nervous tissue that innervates the musculature of the oral sucker, pharynx and body wall. Tissue from the tail, which contains little nervous innervation, has approximately 20 times less serotonin (0.18 ± 0.01 pmol/mg wet wt.). Immunohistochemistry was used to identify serotonin-like immunoreactive cells. Bipolar and multipolar cell bodies in the cerebral ganglia show serotonin-like immunoreactivity. Also evident are serotonin-like immunoreactive processes in the neuropile and in the transverse commissure that concepts the ganglia, and immunoreactive peripheral bipolar cell bodies innervating the musculature of the pharynx and body wall. The cell bodies containing serotonin are organized in bilateral symmetry with homologous cell bodies and processes represented in each ganglion and on both sides of the pharynx.

Original languageEnglish (US)
Pages (from-to)57-62
Number of pages6
JournalBrain research
Issue number1
StatePublished - Oct 25 1988
Externally publishedYes


All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


  • Bilateral symmetry
  • Cerebral ganglion
  • Immunohistochemistry
  • Serotonin

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