Immunolocalization of skeletal matrix proteins in tissue and mineral of the coral Stylophora pistillata

Tali Mass, Jeana L. Drake, Esther C. Peters, Wenge Jiang, Paul Falkowski

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The precipitation and assembly of calcium carbonate skeletons by stony corals is a precisely controlled process regulated by the secretion of an ECM. Recently, it has been reported that the proteome of the skeletal organic matrix (SOM) contains a group of coral acid-rich proteins as well as an assemblage of adhesion and structural proteins, which together, create a framework for the precipitation of aragonite. To date, we are aware of no report that has investigated the localization of individual SOM proteins in the skeleton. In particular, no data are available on the ultrastructural mapping of these proteins in the calcification site or the skeleton. This information is crucial to assessing the role of these proteins in biomineralization. Immunological techniques represent a valuable approach to localize a single component within a calcified skeleton. By using immunogold labeling and immunohistochemical assays, here we show the spatial arrangement of key matrix proteins in tissue and skeleton of the common zooxanthellate coral, Stylophora pistillata. To our knowledge, our results reveal for the first time that, at the nanoscale, skeletal proteins are embedded within the aragonite crystals in a highly ordered arrangement consistent with a diel calcification pattern. In the tissue, these proteins are not restricted to the calcifying epithelium, suggesting that they also play other roles in the coral's metabolic pathways.

Original languageEnglish (US)
Pages (from-to)12728-12733
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number35
DOIs
StatePublished - Sep 2 2014

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Anthozoa
Minerals
Skeleton
Calcium Carbonate
Proteins
Immunologic Techniques
Secretory Pathway
Proteome
Metabolic Networks and Pathways
Epithelium
Acids

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • Actin
  • CARPs
  • Cadherins
  • Carbonic anhydrase

Cite this

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abstract = "The precipitation and assembly of calcium carbonate skeletons by stony corals is a precisely controlled process regulated by the secretion of an ECM. Recently, it has been reported that the proteome of the skeletal organic matrix (SOM) contains a group of coral acid-rich proteins as well as an assemblage of adhesion and structural proteins, which together, create a framework for the precipitation of aragonite. To date, we are aware of no report that has investigated the localization of individual SOM proteins in the skeleton. In particular, no data are available on the ultrastructural mapping of these proteins in the calcification site or the skeleton. This information is crucial to assessing the role of these proteins in biomineralization. Immunological techniques represent a valuable approach to localize a single component within a calcified skeleton. By using immunogold labeling and immunohistochemical assays, here we show the spatial arrangement of key matrix proteins in tissue and skeleton of the common zooxanthellate coral, Stylophora pistillata. To our knowledge, our results reveal for the first time that, at the nanoscale, skeletal proteins are embedded within the aragonite crystals in a highly ordered arrangement consistent with a diel calcification pattern. In the tissue, these proteins are not restricted to the calcifying epithelium, suggesting that they also play other roles in the coral's metabolic pathways.",
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Immunolocalization of skeletal matrix proteins in tissue and mineral of the coral Stylophora pistillata. / Mass, Tali; Drake, Jeana L.; Peters, Esther C.; Jiang, Wenge; Falkowski, Paul.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 35, 02.09.2014, p. 12728-12733.

Research output: Contribution to journalArticle

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