Immunologic studies on nucleic acids and their components II. Reversible inhibition of anti-nucleoside antibodies in aqueous pyridine and its application in antibody purification

M. Z. Humayun; T. M. Jacob

doi:10.1016/0005-2787(74)90011-2

Immunologic studies on nucleic acids and their components II. Reversible inhibition of anti-nucleoside antibodies in aqueous pyridine and its application in antibody purification

M. Z. Humayun, T. M. Jacob

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The effect of aqueous pyridine on a hapten-antihapten system was investigated by the quantitative precipitin reaction and by the membrane filtration method. It was found that dilute solutions of pyridine inhibited the reaction between isopentenyladenosine and its antiserum. Other solvents examined were less effective. The effect of pyridine was reversible at concentrations where complete inhibition occurred, thus indicating its use for the dissociation of antigen-antibody complexes. The inhibitory effect of pyridine was exploited in a single-step purification method for anti-isopentenyladenosine and antideoxy-adenylate antibodies. In addition, generally applicable methods for linking nucleosides and nucleotides to aminoethyl-Sepharose are described.

Original language	English (US)
Pages (from-to)	84-95
Number of pages	12
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	349
Issue number	1
DOIs	https://doi.org/10.1016/0005-2787(74)90011-2
State	Published - Apr 27 1974
Externally published	Yes

All Science Journal Classification (ASJC) codes

Medicine(all)

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10.1016/0005-2787(74)90011-2

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title = "Immunologic studies on nucleic acids and their components II. Reversible inhibition of anti-nucleoside antibodies in aqueous pyridine and its application in antibody purification",

abstract = "The effect of aqueous pyridine on a hapten-antihapten system was investigated by the quantitative precipitin reaction and by the membrane filtration method. It was found that dilute solutions of pyridine inhibited the reaction between isopentenyladenosine and its antiserum. Other solvents examined were less effective. The effect of pyridine was reversible at concentrations where complete inhibition occurred, thus indicating its use for the dissociation of antigen-antibody complexes. The inhibitory effect of pyridine was exploited in a single-step purification method for anti-isopentenyladenosine and antideoxy-adenylate antibodies. In addition, generally applicable methods for linking nucleosides and nucleotides to aminoethyl-Sepharose are described.",

author = "Humayun, {M. Z.} and Jacob, {T. M.}",

note = "Funding Information: We thank Dr J.D. Cherayil for going through the manuscript. This work was partly financed by a grant (FG-In-474) made by the United States Department of Agriculture, Agricultural Research Service, authorized by Public Law 480.",

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doi = "10.1016/0005-2787(74)90011-2",

language = "English (US)",

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Immunologic studies on nucleic acids and their components II. Reversible inhibition of anti-nucleoside antibodies in aqueous pyridine and its application in antibody purification. / Humayun, M. Z.; Jacob, T. M.

In: BBA Section Nucleic Acids And Protein Synthesis, Vol. 349, No. 1, 27.04.1974, p. 84-95.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

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AU - Humayun, M. Z.

AU - Jacob, T. M.

N1 - Funding Information: We thank Dr J.D. Cherayil for going through the manuscript. This work was partly financed by a grant (FG-In-474) made by the United States Department of Agriculture, Agricultural Research Service, authorized by Public Law 480.

PY - 1974/4/27

Y1 - 1974/4/27

N2 - The effect of aqueous pyridine on a hapten-antihapten system was investigated by the quantitative precipitin reaction and by the membrane filtration method. It was found that dilute solutions of pyridine inhibited the reaction between isopentenyladenosine and its antiserum. Other solvents examined were less effective. The effect of pyridine was reversible at concentrations where complete inhibition occurred, thus indicating its use for the dissociation of antigen-antibody complexes. The inhibitory effect of pyridine was exploited in a single-step purification method for anti-isopentenyladenosine and antideoxy-adenylate antibodies. In addition, generally applicable methods for linking nucleosides and nucleotides to aminoethyl-Sepharose are described.

AB - The effect of aqueous pyridine on a hapten-antihapten system was investigated by the quantitative precipitin reaction and by the membrane filtration method. It was found that dilute solutions of pyridine inhibited the reaction between isopentenyladenosine and its antiserum. Other solvents examined were less effective. The effect of pyridine was reversible at concentrations where complete inhibition occurred, thus indicating its use for the dissociation of antigen-antibody complexes. The inhibitory effect of pyridine was exploited in a single-step purification method for anti-isopentenyladenosine and antideoxy-adenylate antibodies. In addition, generally applicable methods for linking nucleosides and nucleotides to aminoethyl-Sepharose are described.

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Immunologic studies on nucleic acids and their components II. Reversible inhibition of anti-nucleoside antibodies in aqueous pyridine and its application in antibody purification

Abstract

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