Immunoprecipitation of human H-Y antigen

John L. Hall, Yuri Bushkin, Stephen S. Wachtel

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

In the absence of beta-2-microglobulin and MHC-determined cell surface antigens, cultured cells of the Burkitt lymphoma, Daudi, secrete testis-inducing H-Y antigen into the surrounding medium. We have precipitated Daudi-secreted H-Y antigen by two methods, one using mouse H-Y antibody and goat anti-mouse Ig, and the other using mouse H-Y antibody and Sepharose beads coated with protein A. The estimated molecular weight of the specific immunoprecipitate was 15,000-18,000 Daltons.

Original languageEnglish (US)
Pages (from-to)34-36
Number of pages3
JournalHuman Genetics
Volume58
Issue number1
DOIs
StatePublished - Aug 1981

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

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