Improved E. coli erythromycin a production through the application of metabolic and bioprocess engineering

Haoran Zhang, Karin Skalina, Ming Jiang, Blaine A. Pfeifer

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


In this report, small-scale culture and bioreactor experiments were used to compare and improve the heterologous production of the antibiotic erythromycin A across a series of engineered prototype Escherichia coli strains. The original strain, termed BAP1(pBPJW130, pBPJW144, pHZT1, pHZT2, pHZT4, pGro7), was designed to allow full erythromycin A biosynthesis from the exogenous addition of propionate. This strain was then compared against two alternatives hypothesized to increase final product titer. Strain TB3(pBPJW130, pBPJW144, pHZT1, pHZT2, pHZT4, pGro7) is a derivative of BAP1 designed to increase biosynthetic pathway carbon flow as a result of a ygfH deletion; whereas, strain TB3(pBPJW130, pBPJW144, pHZT1, pHZT2, pHZT4-2, pGro7) provided an extra copy of a key deoxysugar glycosyltransferase gene. Production was compared across the three strains with TB3(pBPJW130, pBPJW144, pHZT1, pHZT2, pHZT4, pGro7) showing significant improvement in erythronolide B (EB), 3-mycarosylerythronolide B (MEB), and erythromycin A titers. This strain was further tested in the context of batch bioreactor production experiments with time-course titers leveling at 4 mg/L, representing an approximately sevenfold increase in final erythromycin A titer.

Original languageEnglish (US)
Pages (from-to)292-296
Number of pages5
JournalBiotechnology Progress
Issue number1
StatePublished - Jan 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology


  • E. coli
  • Erythromycin A
  • Heterologous biosynthesis
  • Metabolic and process engineering


Dive into the research topics of 'Improved E. coli erythromycin a production through the application of metabolic and bioprocess engineering'. Together they form a unique fingerprint.

Cite this