Improved expression of streptomycin resistance in plants due to a deletion in the streptomycin phosphotransferase coding sequence

Pal Maliga, Zora Svab, Elisabeth C. Harper, Jonathan D G Jones

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Previous studies have shown that a chimeric streptomycin phosphotransferase (SPT) gene can function as a dominant marker for plant cell transformation. The SPT marker previously described by Jones and co-workers has a limited value since it conferred a useful level of resistance only to a fraction (10%) of Nicotiana plumbaginifolia transgenic lines. Expression of resistance was species specific: no such resistant transformants were found in N. tabacum. In this paper we describe an improved SPT construct that utilizes a mutant Tn 5 SPT gene. The mutant gene, SPT*, encodes a protein with a two amino acid deletion close to its COOH-terminus. In N. tabacum cell culture the efficiency of transformation with the improved streptomycin resistance marker was comparable to kanamycin resistance. When the chimeric SPT* gene was introduced linked to a kanamycin resistance gene, streptomycin resistance was expressed in most of the transgenic N. tabacum lines.

Original languageEnglish (US)
Pages (from-to)456-459
Number of pages4
JournalMgg Molecular & General Genetics
Volume214
Issue number3
DOIs
StatePublished - Nov 1 1988
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

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