Improved high-speed capillary electrophoresis system using a short capillary and picoliter-scale translational spontaneous injection

Ting Zhang, Jinglin Fu, Qun Fang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Here, we describe an improved high-speed CE (HSCE) system using a short capillary and translational spontaneous sample injection. Several important factors for consideration in system design as well as various factors influencing the performance of the HSCE system were investigated in detail. The performance of this HSCE system was demonstrated in electrophoretic separation of FITC-labeled amino acids. Under optimized conditions, baseline separation of eight amino acids and FITC were achieved in 21 s with the plate heights ranging from 0.20 to 0.31 μm, corresponding to a separation rate up to 20700 theoretical plates per second. The separation speed and efficiency of the optimized high-speed CE system are comparable to or even better than those reported in microchip-based CE systems.

Original languageEnglish (US)
Pages (from-to)2361-2369
Number of pages9
JournalELECTROPHORESIS
Volume35
Issue number16
DOIs
StatePublished - Jan 1 2014

Fingerprint

Capillary electrophoresis
Fluorescein-5-isothiocyanate
Capillary Electrophoresis
Amino Acids
Injections
Systems analysis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry

Keywords

  • Amino acids
  • High-speed CE
  • Short capillary
  • Spontaneous sample injection

Cite this

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abstract = "Here, we describe an improved high-speed CE (HSCE) system using a short capillary and translational spontaneous sample injection. Several important factors for consideration in system design as well as various factors influencing the performance of the HSCE system were investigated in detail. The performance of this HSCE system was demonstrated in electrophoretic separation of FITC-labeled amino acids. Under optimized conditions, baseline separation of eight amino acids and FITC were achieved in 21 s with the plate heights ranging from 0.20 to 0.31 μm, corresponding to a separation rate up to 20700 theoretical plates per second. The separation speed and efficiency of the optimized high-speed CE system are comparable to or even better than those reported in microchip-based CE systems.",
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Improved high-speed capillary electrophoresis system using a short capillary and picoliter-scale translational spontaneous injection. / Zhang, Ting; Fu, Jinglin; Fang, Qun.

In: ELECTROPHORESIS, Vol. 35, No. 16, 01.01.2014, p. 2361-2369.

Research output: Contribution to journalArticle

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