TY - JOUR
T1 - Improved M13 phage cloning vectors and host strains
T2 - nucleotide sequences of the M13mpl8 and pUC19 vectors
AU - Yanisch-Perron, Celeste
AU - Vieira, Jeffrey
AU - Messing, Joachim
N1 - Funding Information:
We thank Kris Kohn, Tammy Krogmann, Mike Zarowitz, and StephanieY oung for help in preparingth em anuscriptD, rs. J. Felton,T .Baldwin and P. Stragierf or makingr esultsa vailablet o us prior to publication,a nd Betsy Kren and James Fuchs for helpfuld iscussionsT. he work was supported by the Departmento f Energy DE-FG02-84ER13210a nd NIH GM31499.J .V. is supported by the NIH trainingg rantN o. ST32 GM107094.
PY - 1985
Y1 - 1985
N2 - Three kinds of improvements have been introduced into the M13-based cloning systems. (1) New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors. Mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences. A new suppressorless strain facilitates the cloning of selected recombinants. (2) The complete nucleotide sequences of the M 13mp and pUC vectors have been compiled from a number of sources, including the sequencing of selected segments. The M13mp18 sequence is revised to include the G-to-T substitution in its gene II at position 6 125 bp (in M13) or 6967 bp in M13mp18. (3) M13 clones suitable for sequencing have been obtained by a new method of generating unidirectional progressive deletions from the polycloning site using exonucleases HI and VII.
AB - Three kinds of improvements have been introduced into the M13-based cloning systems. (1) New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 and the high-copy-number pUC-plasmid cloning vectors. Mutations introduced into these strains improve cloning of unmodified DNA and of repetitive sequences. A new suppressorless strain facilitates the cloning of selected recombinants. (2) The complete nucleotide sequences of the M 13mp and pUC vectors have been compiled from a number of sources, including the sequencing of selected segments. The M13mp18 sequence is revised to include the G-to-T substitution in its gene II at position 6 125 bp (in M13) or 6967 bp in M13mp18. (3) M13 clones suitable for sequencing have been obtained by a new method of generating unidirectional progressive deletions from the polycloning site using exonucleases HI and VII.
KW - Recombinant DNA
KW - molecular cloning
KW - polycloning sites
KW - progressive deletions
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U2 - 10.1016/0378-1119(85)90120-9
DO - 10.1016/0378-1119(85)90120-9
M3 - Article
C2 - 2985470
AN - SCOPUS:0021943518
VL - 33
SP - 103
EP - 119
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1
ER -