Improved method for simultaneous determination of alcohols, volatile fatty acids, lactic acid or 2,3-butanediol in biological samples

M. J. Teunissen, S. A.E. Marras, H. J.M. Op den Camp, G. D. Vogels

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

A rapid and sample procedure was developed to determine, by gas-liquid chromatography, the concentrations of C2C4 alcohols, C2C6 volatile fatty acids (VFA) and lactic acid or 2,3-butanediol in fermentation liquids. both lactic acid and 2,3-butanediol are oxidized to acetaldehyde by periodic acid and acetaldehyde was eluted before ethanol. A complete separation of the alcohols and acids was performed in <15 min on a column packed with 80 100 Chromosorb WAW, having GP 10% SP-1200/1% H3PO4 as the liquid phase. The method was suitable for the analysis of rumen fluid and fermentation products from microbial cultures. The detection limits for all compounds were <0.13 nmol · injection-1.

Original languageEnglish (US)
Pages (from-to)247-254
Number of pages8
JournalJournal of Microbiological Methods
Volume10
Issue number4
DOIs
StatePublished - Dec 1989
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

Keywords

  • 2,3-Butanediol
  • Alcohol
  • Gas-liquid chromatography
  • Lactic acid
  • Volatile fatty acids

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