Improved method for simultaneous determination of alcohols, volatile fatty acids, lactic acid or 2,3-butanediol in biological samples

M. J. Teunissen; S. A.E. Marras; H. J.M. Op den Camp; G. D. Vogels

doi:10.1016/0167-7012(89)90012-2

Improved method for simultaneous determination of alcohols, volatile fatty acids, lactic acid or 2,3-butanediol in biological samples

M. J. Teunissen, S. A.E. Marras, H. J.M. Op den Camp, G. D. Vogels

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

A rapid and sample procedure was developed to determine, by gas-liquid chromatography, the concentrations of C₂C₄ alcohols, C₂C₆ volatile fatty acids (VFA) and lactic acid or 2,3-butanediol in fermentation liquids. both lactic acid and 2,3-butanediol are oxidized to acetaldehyde by periodic acid and acetaldehyde was eluted before ethanol. A complete separation of the alcohols and acids was performed in <15 min on a column packed with 80 100 Chromosorb WAW, having GP 10% SP-1200/1% H₃PO₄ as the liquid phase. The method was suitable for the analysis of rumen fluid and fermentation products from microbial cultures. The detection limits for all compounds were <0.13 nmol · injection^-1.

Original language	English (US)
Pages (from-to)	247-254
Number of pages	8
Journal	Journal of Microbiological Methods
Volume	10
Issue number	4
DOIs	https://doi.org/10.1016/0167-7012(89)90012-2
State	Published - Dec 1989
Externally published	Yes

All Science Journal Classification (ASJC) codes

Microbiology
Molecular Biology
Microbiology (medical)

Keywords

2,3-Butanediol
Alcohol
Gas-liquid chromatography
Lactic acid
Volatile fatty acids

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10.1016/0167-7012(89)90012-2

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title = "Improved method for simultaneous determination of alcohols, volatile fatty acids, lactic acid or 2,3-butanediol in biological samples",

abstract = "A rapid and sample procedure was developed to determine, by gas-liquid chromatography, the concentrations of C2C4 alcohols, C2C6 volatile fatty acids (VFA) and lactic acid or 2,3-butanediol in fermentation liquids. both lactic acid and 2,3-butanediol are oxidized to acetaldehyde by periodic acid and acetaldehyde was eluted before ethanol. A complete separation of the alcohols and acids was performed in <15 min on a column packed with 80 100 Chromosorb WAW, having GP 10% SP-1200/1% H3PO4 as the liquid phase. The method was suitable for the analysis of rumen fluid and fermentation products from microbial cultures. The detection limits for all compounds were <0.13 nmol · injection-1.",

keywords = "2,3-Butanediol, Alcohol, Gas-liquid chromatography, Lactic acid, Volatile fatty acids",

author = "Teunissen, {M. J.} and Marras, {S. A.E.} and {Op den Camp}, {H. J.M.} and Vogels, {G. D.}",

year = "1989",

month = dec,

doi = "10.1016/0167-7012(89)90012-2",

language = "English (US)",

volume = "10",

pages = "247--254",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

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TY - JOUR

T1 - Improved method for simultaneous determination of alcohols, volatile fatty acids, lactic acid or 2,3-butanediol in biological samples

AU - Teunissen, M. J.

AU - Marras, S. A.E.

AU - Op den Camp, H. J.M.

AU - Vogels, G. D.

PY - 1989/12

Y1 - 1989/12

N2 - A rapid and sample procedure was developed to determine, by gas-liquid chromatography, the concentrations of C2C4 alcohols, C2C6 volatile fatty acids (VFA) and lactic acid or 2,3-butanediol in fermentation liquids. both lactic acid and 2,3-butanediol are oxidized to acetaldehyde by periodic acid and acetaldehyde was eluted before ethanol. A complete separation of the alcohols and acids was performed in <15 min on a column packed with 80 100 Chromosorb WAW, having GP 10% SP-1200/1% H3PO4 as the liquid phase. The method was suitable for the analysis of rumen fluid and fermentation products from microbial cultures. The detection limits for all compounds were <0.13 nmol · injection-1.

AB - A rapid and sample procedure was developed to determine, by gas-liquid chromatography, the concentrations of C2C4 alcohols, C2C6 volatile fatty acids (VFA) and lactic acid or 2,3-butanediol in fermentation liquids. both lactic acid and 2,3-butanediol are oxidized to acetaldehyde by periodic acid and acetaldehyde was eluted before ethanol. A complete separation of the alcohols and acids was performed in <15 min on a column packed with 80 100 Chromosorb WAW, having GP 10% SP-1200/1% H3PO4 as the liquid phase. The method was suitable for the analysis of rumen fluid and fermentation products from microbial cultures. The detection limits for all compounds were <0.13 nmol · injection-1.

KW - 2,3-Butanediol

KW - Alcohol

KW - Gas-liquid chromatography

KW - Lactic acid

KW - Volatile fatty acids

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U2 - 10.1016/0167-7012(89)90012-2

DO - 10.1016/0167-7012(89)90012-2

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JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

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