Improved Plasmodium berghei lines for conditional mutagenesis

Dhruv Panchal, Kavitha Govindasamy, Arti Rana, Purnima Bhanot

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Conditional mutagenesis is a powerful tool for genetic analysis in Plasmodium berghei. It allows the study of proteins that function both during the parasite's pre-erythocytic and erythrocytic development. Currently available parasite lines used for conditional mutagenesis were constructed in the NK65 strain, and express a DNA recombinase under the control of pre-erythrocytic stage-specific promoters. However, the integration of the recombinase in these lines is unstable leading to inconsistent excision of the target gene. We describe improved lines of P. berghei with stably integrated DNA recombinase that allow efficient, stage-specific excision of target genes in the widely used ANKA strain.

Original languageEnglish (US)
Pages (from-to)52-54
Number of pages3
JournalMolecular and Biochemical Parasitology
Volume184
Issue number1
DOIs
StatePublished - Jul 1 2012

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Molecular Biology

Keywords

  • Conditional mutagenesis
  • Flp-FRT
  • Genetic analysis
  • Plasmodium berghei

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