In situ detection of early replication phases of a gemini virus in legume protoplasts

Dale M. Steffensen; H. J. Wilson; Robert M. Goodman

doi:10.1007/BF00272783

In situ detection of early replication phases of a gemini virus in legume protoplasts

Dale M. Steffensen, H. J. Wilson, Robert M. Goodman

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Protoplasts of Phaseolus vulgaris L. (Top Crop), infected with bean golden mosaic virus, were isolated and fixed by various methods for in situ hybridization. An iodine-125 labeled probe was made from the replicative form of the virus. The localization and quantitation was done by autoradiography. Cell wall removal lowered the background and allowed a more accurate analysis. RNase was used to eliminate the possibility of hybrids to RNA. The evidence suggests a sequence of virus movements starting from rough endoplasm reticulum, moving to the nuclear membrane, and finally with the highest concentration inside the nucleus.

Original language	English (US)
Pages (from-to)	462-465
Number of pages	4
Journal	Plant Cell Reports
Volume	6
Issue number	6
DOIs	https://doi.org/10.1007/BF00272783
State	Published - Dec 1987
Externally published	Yes

All Science Journal Classification (ASJC) codes

Agronomy and Crop Science
Plant Science

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10.1007/BF00272783

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title = "In situ detection of early replication phases of a gemini virus in legume protoplasts",

abstract = "Protoplasts of Phaseolus vulgaris L. (Top Crop), infected with bean golden mosaic virus, were isolated and fixed by various methods for in situ hybridization. An iodine-125 labeled probe was made from the replicative form of the virus. The localization and quantitation was done by autoradiography. Cell wall removal lowered the background and allowed a more accurate analysis. RNase was used to eliminate the possibility of hybrids to RNA. The evidence suggests a sequence of virus movements starting from rough endoplasm reticulum, moving to the nuclear membrane, and finally with the highest concentration inside the nucleus.",

author = "Steffensen, {Dale M.} and Wilson, {H. J.} and Goodman, {Robert M.}",

year = "1987",

month = dec,

doi = "10.1007/BF00272783",

language = "English (US)",

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pages = "462--465",

journal = "Plant Cell Reports",

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T1 - In situ detection of early replication phases of a gemini virus in legume protoplasts

AU - Steffensen, Dale M.

AU - Wilson, H. J.

AU - Goodman, Robert M.

PY - 1987/12

Y1 - 1987/12

N2 - Protoplasts of Phaseolus vulgaris L. (Top Crop), infected with bean golden mosaic virus, were isolated and fixed by various methods for in situ hybridization. An iodine-125 labeled probe was made from the replicative form of the virus. The localization and quantitation was done by autoradiography. Cell wall removal lowered the background and allowed a more accurate analysis. RNase was used to eliminate the possibility of hybrids to RNA. The evidence suggests a sequence of virus movements starting from rough endoplasm reticulum, moving to the nuclear membrane, and finally with the highest concentration inside the nucleus.

AB - Protoplasts of Phaseolus vulgaris L. (Top Crop), infected with bean golden mosaic virus, were isolated and fixed by various methods for in situ hybridization. An iodine-125 labeled probe was made from the replicative form of the virus. The localization and quantitation was done by autoradiography. Cell wall removal lowered the background and allowed a more accurate analysis. RNase was used to eliminate the possibility of hybrids to RNA. The evidence suggests a sequence of virus movements starting from rough endoplasm reticulum, moving to the nuclear membrane, and finally with the highest concentration inside the nucleus.

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In situ detection of early replication phases of a gemini virus in legume protoplasts

Abstract

All Science Journal Classification (ASJC) codes

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