In vitro and in vivo evaluation of albumin synthesis rate of porcine hepatocytes in a flat-plate bioreactor

Masaya Shito, Nam Heui Kim, Harihara Baskaran, Arno W. Tilles, Ronald G. Tompkins, Martin L. Yarmush, Mehmet Toner

Research output: Contribution to journalReview articlepeer-review

39 Scopus citations

Abstract

Several configurations of extracorporeal bioartificial liver devices have been developed for the potential treatment of fulminant hepatic failure or as a bridge to liver transplantation. Recently, we developed a microchannel flat-plate bioreactor with an internal membrane oxygenator in which porcine hepatocytes are cultured as a monolayer on the bottom glass surface. In the present study, we investigated synthetic function of porcine hepatocytes in the bioreactor in both in vitro and in vivo flow circuit models. In vitro, albumin synthesis was stable in the bioreactor for up to 4 days of perfusion. In vivo, with the extracorporeal connection of the bioreactor to rat vasculature, porcine albumin was detectable for 24 h in the rat plasma. We also developed a simple mathematical model to predict the in vivo porcine albumin concentration in rat plasma. These results indicate that this configuration of a microchannel flat-plate bioreactor has potential as a liver support device and warrants further investigation.

Original languageEnglish (US)
Pages (from-to)571-578
Number of pages8
JournalArtificial Organs
Volume25
Issue number7
DOIs
StatePublished - 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Keywords

  • Albumin production
  • Bioartificial liver
  • Flat-plate bioreactor
  • In vivo perfusion
  • Pharmacokinetics
  • Porcine hepatocyte culture

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