In vitro explant culture and related protocols for the study of mouse retinal development

Kangxin Jin, Mengqing Xiang

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Scopus citations

Abstract

The mouse retina is composed of many cell types and subtypes with distinct morphology and function; how these cells are differentiated from the multipotent progenitors is still largely unknown. Retinal in vitro explant culture has proven to be a useful tool to study the molecular and cellular mechanisms underlying retinal development. Here, we provide detailed descriptions about how to prepare retroviruses, dissect retinal cups, perform in vitro explant culture, and collect explant samples.

Original languageEnglish (US)
Title of host publicationRetinal Development
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages155-165
Number of pages11
ISBN (Print)9781617798474
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume884
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Development
  • Differentiation
  • Electroporation
  • Explant culture
  • Retina
  • Retrovirus

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