In vitro investigation of streptokinase activity using Sonoclot coagulation analysis

B. A. Bhatt, P. C. Uppuluri, D. Kristol, R. R. Arora, C. R. Spillert

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations


Streptokinase (SK) therapy has become a common clinical intervention in the treatment of acute coronary syndromes. Recent clinical trials indicate that adjuvant treatment with low molecular weight heparin (LMWH) or bivalirudin (BV) may attenuate fibrinolytic-induced procoagulant activity. We investigated the effects of SK with LMWH and BV on the clotting of whole blood using a Sonoclot® Coagulation Analyzer. Human citrated whole blood was spiked with SK and LMWH at final concentrations of control, 5 U/mL SK, 0.2 U/mL LMWH, and 5 U/mL SK:0.2 U/mL LMWH. The samples (n=10) were incubated at 37°C for 10 minutes and recalcified with CaCl2 to initiate clotting. Tracings were obtained using the Sonoclot Signature Viewer™ software, and the clotting time (CT) and clot viscosity were noted. The experiment was repeated (n=8) using 5 μg/mL BV in place of LMWH. The Sonoclot tracings provide a whole-picture analysis of the effects of SK and adjuvant anticoagulants on haemostatic and fibrinolytic mechanisms. Implementation of coagulation monitoring protocols for SK therapy may aid in clinical diagnosis.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE 29th Annual Northeast Bioengineering Conference
EditorsStanley Reisman, Richard Foulds, Bruno Mantilla
PublisherInstitute of Electrical and Electronics Engineers Inc.
Number of pages2
ISBN (Electronic)0780377672
StatePublished - 2003
Event29th IEEE Annual Northeast Bioengineering Conference, NEBC 2003 - Newark, United States
Duration: Mar 22 2003Mar 23 2003

Publication series

NameProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
ISSN (Print)1071-121X
ISSN (Electronic)2160-7001


Other29th IEEE Annual Northeast Bioengineering Conference, NEBC 2003
Country/TerritoryUnited States

All Science Journal Classification (ASJC) codes

  • Bioengineering


  • Blood
  • Clinical diagnosis
  • Clinical trials
  • Coagulation
  • Humans
  • In vitro
  • Medical treatment
  • Monitoring
  • Protocols
  • Viscosity


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