In vitro investigation of streptokinase activity using sonoclot coagulation analysis

Biren A. Bhatt, Pranay C. Uppuluri, David Kristol, Rohit R. Arora, Charles Spillert

Research output: Contribution to conferencePaper

Abstract

Streptoklnase (SK) therapy has become a common clinical intervention in the treatment of acute coronary syndromes. Recent clinical trials indicate that adjuvant treatment with low molecular weight heparin (LMWH) or bivalirudin (BV) may attenuate fibrinolytic-induced procoagulant activity. We investigated the effects of SK with LMWH and BV on the clotting of whole blood using a Sonoclot® Coagulation Analyzer. Human citrated whole blood was spiked with SK and LMWH at final concentrations of control, 5 U/mL SK, 0.2 U/mL LMWH, and 5 U/mL SK:0.2 U/mL LMWH. The samples (n=10) were incubated at 37°C for 10 minutes and recalcified with CaCl2 to initiate clotting. Tracings were obtained using the Sonoclot Signature Viewer software, and the clotting time (CT) and maximum clot viscosity were noted. The experiment was repeated (n=8) using 5 μg/mL BV in place of LMWH. The Sonoclot tracings provide a whole-picture analysis of the effects of SK and adjuvant anticoagulants on haemostatic and fibrinolytic mechanisms. Implementation of coagulation monitoring protocols for SK therapy may aid in clinical diagnosis.

Original languageEnglish (US)
Pages253-254
Number of pages2
Publication statusPublished - Aug 22 2003
EventProceedings of the IEEE 29th Annual Northeast Bioengineering Conference - Newark, NJ, United States
Duration: Mar 22 2003Mar 23 2003

Other

OtherProceedings of the IEEE 29th Annual Northeast Bioengineering Conference
CountryUnited States
CityNewark, NJ
Period3/22/033/23/03

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All Science Journal Classification (ASJC) codes

  • Bioengineering

Cite this

Bhatt, B. A., Uppuluri, P. C., Kristol, D., Arora, R. R., & Spillert, C. (2003). In vitro investigation of streptokinase activity using sonoclot coagulation analysis. 253-254. Paper presented at Proceedings of the IEEE 29th Annual Northeast Bioengineering Conference, Newark, NJ, United States.