In vitro investigation of the procoagulant properties of silver ion

Biren A. Bhatt, David Kristol, Charles Spillert

Research output: Contribution to conferencePaper

Abstract

Silver ion, a potent antimicrobial and procoagulant, is hypothesized to achieve its haemostatic effect by binding anticoagulant proteins. We performed in vitro studies to gain more insight into its mechanism of action. 10 μL of 5% AgNO 3 was added to 1 mL human plasma (n=4), forming a precipitate. The precipitate was recovered through centrifugation and iteratively washed with saline. The supernatant(s) and precipitate were mixed with Coomassie blue stain and protein content was measured via spectrophotometric absorbance. An analysis of variance indicated significant (p<0.0001) differences between all groups. Multiple comparison tests showed a significant (p<0.001) increase in absorbance from the final wash and dye blank to the precipitate, indicating the presence of proteins in the precipitate. Silver ion may be precipitating anticoagulant proteins as its mechanism of action.

Original languageEnglish (US)
Pages178-179
Number of pages2
Publication statusPublished - Jun 22 2004
EventProceedings of the IEEE 30th Annual Northeast Bioengineering Conference - Springfield, MA, United States
Duration: Apr 17 2004Apr 18 2004

Other

OtherProceedings of the IEEE 30th Annual Northeast Bioengineering Conference
CountryUnited States
CitySpringfield, MA
Period4/17/044/18/04

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All Science Journal Classification (ASJC) codes

  • Bioengineering

Cite this

Bhatt, B. A., Kristol, D., & Spillert, C. (2004). In vitro investigation of the procoagulant properties of silver ion. 178-179. Paper presented at Proceedings of the IEEE 30th Annual Northeast Bioengineering Conference, Springfield, MA, United States.