@inbook{5a97cc72970e4d8ba88b87f3e9110382,
title = "In vitro modulation of endogenous alternative splicing using splice-switching antisense oligonucleotides",
abstract = "Regulation of alternative splicing can be harnessed by antisense-based compounds to control gene expression. Antisense-mediated splicing interference has become a valuable molecular tool to modulate endogenous alternative splicing patterns, to correct cryptic or aberrant splicing, to reduce gene expression by triggering nonsense-mediated mRNA decay, and to activate intronic polyadenylation, both in vitro and in vivo. Here, we describe methods to induce and analyze the modulation of RNA processing, using modified splice-switching antisense oligonucleotides, such as phosphorodiamidate morpholino (PMO).",
keywords = "Antisense-oligonucleotide, MRNA, Morpholino, Polyadenylation, Splicing",
author = "Park, {Jeong Eun} and Luca Cartegni",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media LLC 2017.",
year = "2017",
doi = "10.1007/978-1-4939-7204-3_4",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "39--52",
booktitle = "Methods in Molecular Biology",
}