In vitro packaging of individual genomic segments of bacteriophage φ6 RNA: Serial dependence relationships

Xueying Qiao, Gregory Casini, Jian Qiao, Leonard Mindich

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Bacteriophage φ6 has a genome of three segments of double-stranded RNA enclosed in a procapsid composed of four different proteins. The preformed procapsid is capable of packaging plus-strand transcripts of the genomic segments in an in vitro reaction. The packaging of the three segments shows a strong order of dependence in that segment S packages alone, but segment M requires S and segment L requires S and M for efficient packaging. Packaging of individual segments is dependent on unique packaging sequences of about 200 nucleotides near the 5' ends of the segments. Deletions that invade these regions destroy packaging competence for the particular segment and for the dependent segments as well. In the presence of 2 mM phosphate and at magnesium ion concentrations above 4 mM, packaging becomes progressively more independent and ultimately nonspecific with respect to φ6 sequences.

Original languageEnglish (US)
Pages (from-to)2926-2931
Number of pages6
JournalJournal of virology
Volume69
Issue number5
DOIs
StatePublished - May 1995

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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