Abstract
The construction of a tissue engineered liver requires the ready availability of an immunocompatible cell source capable of replacing lost physiological functions. Primary autologous hepatocytes would be an ideal choice for such a device, however, it is not yet known how to induce proliferation of these cells in vitro. We investigated the effect of culture medium containing known liver regeneration factors (HGF, EGF, VEGF, IL-6) pre-conditioned by culture with nonparenchymal cells (NPCs) on the proliferation of primary hepatocytes in collagen sandwich culture. Morphological evidence indicated an increase in the density of hepatocytes when supplemented with proliferation factor-conditioned NPC media.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 780-781 |
| Number of pages | 2 |
| Journal | Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings |
| Volume | 1 |
| State | Published - 2002 |
| Externally published | Yes |
| Event | Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States Duration: Oct 23 2002 → Oct 26 2002 |
All Science Journal Classification (ASJC) codes
- Signal Processing
- Biomedical Engineering
- Computer Vision and Pattern Recognition
- Health Informatics
Keywords
- Nonparenchymal cells
- Primary hepatocyte
- Proliferation
- Regeneration