In vitro transformation by bromodeoxyuridine and X irradiation in C3H 10T 1/2 cells

G. P. Raaphorst, E. I. Azzam, J. Borsa, M. D. Sargent

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The effect of 10-5 M bromodeoxyuridine (BrdUrd) substitution in C3H 10T 1/2 cells was evaluated. Cellular toxicity increased rapidly for BrdUrd exposure times that were longer than the population doubling time. Radiosensitization by BrdUrd exposure was almost complete after one cell doubling time and was characterized by a decrease in D0 and the survival curve shoulder. Exposure to BrdUrd for one cell doubling time produced only very low transformation levels, but for prolonged BrdUrd exposure times, the transformation frequency per viable cell increased significantly. BrdUrd incorporation also enhanced radiation induction of transformation above the transformation levels resulting from the independent action of X rays or BrdUrd treatment. These results show that BrdUrd is a transforming agent in C3H 10T 1/2 cells and thus may be a carcinogen and that BrdUrd can enhance radiation-induced transformation.

Original languageEnglish (US)
Pages (from-to)279-291
Number of pages13
JournalRadiation Research
Volume101
Issue number2
DOIs
StatePublished - 1985
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

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