In vivo expression of a nonselected gene transferred into murine hematopoietic stem cells by electroporation

R. Narayanan; M. M. Jastreboff; C. F. Chiu; J. R. Bertino

doi:10.1016/S0006-291X(86)80146-2

In vivo expression of a nonselected gene transferred into murine hematopoietic stem cells by electroporation

R. Narayanan, M. M. Jastreboff, C. F. Chiu, J. R. Bertino

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Mouse bone marrow cells were subjected to electroporation in the presence of RSVCAT and SV₂NEO plasmids. CAT activity was detected in the G-418 resistant granulocyte-macrophage colonies. RSVCAT electroporated into primary bone marrow cells, repopulated lethally irradiated mice as demonstrated by the persistence of CAT activity in the hematopoietic tissues showing that electroporation can offer a powerful mode of gene transfer into bone marrow cells.

Original language	English (US)
Pages (from-to)	1018-1024
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	141
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(86)80146-2
State	Published - Dec 30 1986
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/S0006-291X(86)80146-2

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title = "In vivo expression of a nonselected gene transferred into murine hematopoietic stem cells by electroporation",

abstract = "Mouse bone marrow cells were subjected to electroporation in the presence of RSVCAT and SV2NEO plasmids. CAT activity was detected in the G-418 resistant granulocyte-macrophage colonies. RSVCAT electroporated into primary bone marrow cells, repopulated lethally irradiated mice as demonstrated by the persistence of CAT activity in the hematopoietic tissues showing that electroporation can offer a powerful mode of gene transfer into bone marrow cells.",

author = "R. Narayanan and Jastreboff, {M. M.} and Chiu, {C. F.} and Bertino, {J. R.}",

note = "Funding Information: ACKNOWLEDGMENTS: This work was supported by CA 28852 from the National Cancer Institute. R. Narayanan is a recepient of a grant IN-31-28 from the American Cancer Society. M.M. Jastreboff is a Special Fellow at The Leukemia Society of America. J.R. Bertino is an American Cancer Society Professor. C.F. Chiu is a Fellow of Merck Foundation. Address reprints requests to R. Narayanan. We thank Ms. Barbara Brown for helpful suggestions and Ms. Jeanine Narayanan for editorial assistance.",

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doi = "10.1016/S0006-291X(86)80146-2",

language = "English (US)",

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T1 - In vivo expression of a nonselected gene transferred into murine hematopoietic stem cells by electroporation

AU - Narayanan, R.

AU - Jastreboff, M. M.

AU - Chiu, C. F.

AU - Bertino, J. R.

N1 - Funding Information: ACKNOWLEDGMENTS: This work was supported by CA 28852 from the National Cancer Institute. R. Narayanan is a recepient of a grant IN-31-28 from the American Cancer Society. M.M. Jastreboff is a Special Fellow at The Leukemia Society of America. J.R. Bertino is an American Cancer Society Professor. C.F. Chiu is a Fellow of Merck Foundation. Address reprints requests to R. Narayanan. We thank Ms. Barbara Brown for helpful suggestions and Ms. Jeanine Narayanan for editorial assistance.

PY - 1986/12/30

Y1 - 1986/12/30

N2 - Mouse bone marrow cells were subjected to electroporation in the presence of RSVCAT and SV2NEO plasmids. CAT activity was detected in the G-418 resistant granulocyte-macrophage colonies. RSVCAT electroporated into primary bone marrow cells, repopulated lethally irradiated mice as demonstrated by the persistence of CAT activity in the hematopoietic tissues showing that electroporation can offer a powerful mode of gene transfer into bone marrow cells.

AB - Mouse bone marrow cells were subjected to electroporation in the presence of RSVCAT and SV2NEO plasmids. CAT activity was detected in the G-418 resistant granulocyte-macrophage colonies. RSVCAT electroporated into primary bone marrow cells, repopulated lethally irradiated mice as demonstrated by the persistence of CAT activity in the hematopoietic tissues showing that electroporation can offer a powerful mode of gene transfer into bone marrow cells.

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ER -

In vivo expression of a nonselected gene transferred into murine hematopoietic stem cells by electroporation

Abstract

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