In vivo expression of a nonselected gene transferred into murine hematopoietic stem cells by electroporation

R. Narayanan, M. M. Jastreboff, C. F. Chiu, J. R. Bertino

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Mouse bone marrow cells were subjected to electroporation in the presence of RSVCAT and SV2NEO plasmids. CAT activity was detected in the G-418 resistant granulocyte-macrophage colonies. RSVCAT electroporated into primary bone marrow cells, repopulated lethally irradiated mice as demonstrated by the persistence of CAT activity in the hematopoietic tissues showing that electroporation can offer a powerful mode of gene transfer into bone marrow cells.

Original languageEnglish (US)
Pages (from-to)1018-1024
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume141
Issue number3
DOIs
StatePublished - Dec 30 1986
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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