In vivo induction of tyrosylprotein sulfotransferase by ethanol: Role of increased enzyme synthesis

Patalapati Ramaprasad, Chinnaswamy Kasinathan

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Tyrosine sulfation is a posttranslational modification involved in the synthesis, secretion, and biological activity of proteins and peptides. Our previous studies have demonstrated that the enzyme activity was induced by ethanol. In the present work, the induction was studied in detail. Initial experiments were conducted to examine the time course of tyrosylprotein sulfotransferase (TPST) induction in rats pair-fed liquid diets containing either ethanol or carbohydrate substitute (controls). Marked elevation of TPST activity (3-fold) was measured on day 10 in the liver and gastric mucosa of ethanol-fed rats. Ethanol-mediated enhancement was also noticed by Western-blot analysis with anti-TPST antibody in both the liver and gastric mucosa on days 5 and 10. We then determined the steady-state TPST protein turnover in ethanol-fed and control animals that were given 35S-methionine after 10 days of pair-feeding with liquid diet. The rates of TPST synthesis assessed by measuring initial rates of incorporation of 35S-methionine into TPST was increased in the liver and gastric mucosa of animals fed with ethanol. Monophasic exponential decay curves showed that TPST protein half- lives for liver (control: 34 hr, ethanol: 32 hr) and gastric mucosa (control: 52 hr, ethanol: 48 hr) did not differ between control and ethanol groups. Our overall results indicate that the in vivo induction of TPST by ethanol involves increased enzyme synthesis rather than decreased enzyme degradation.

Original languageEnglish (US)
Pages (from-to)1120-1124
Number of pages5
JournalAlcoholism: Clinical and Experimental Research
Volume22
Issue number5
DOIs
StatePublished - Aug 1998

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Toxicology
  • Psychiatry and Mental health

Keywords

  • Anti-TPST Antibody
  • Enzyme Turnover
  • Ethanol Pair-feeding
  • S-Methionine
  • Tyrosylprotein Sulfotransferase

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