In vivo methods to assess polyadenylation efficiency

Lisa K. Hague, Tyra Hall-Pogar, Carol S. Lutz

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Mammalian gene expression can be regulated through various post-transcriptional events, including altered mRNA stability, translational control, and RNA-processing events such as 3′-end formation or polyadenylation (pA). It has become clear in recent years that pA is governed by several core sequence elements and often regulated by additional auxiliary sequence elements. These regulatory events are frequently not reproducible in in vitro assays. Therefore, in vivo methods to measure mRNA pA were developed to meet this need and are described here.

Original languageEnglish (US)
Title of host publicationPost-Transcriptional Gene Regulation
EditorsJeffrey Wilusz
Pages171-185
Number of pages15
DOIs
StatePublished - 2008

Publication series

NameMethods in Molecular Biology
Volume419
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • 3′ UTR
  • Cis-acting core and auxiliary elements
  • Luciferase assays
  • Polyadenylation
  • RNase protection assays
  • Transfection

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