TY - JOUR
T1 - Inactivation and removal of Enterobacter aerogenes biofilm in a model piping system using plasma-activated water (PAW)
AU - Tan, Juzhong
AU - Karwe, Mukund V.
N1 - Funding Information:
Authors are thankful to Dr. Donald W. Schaffner and his students the Department of Food Science at Rutgers University, for providing microbial culture, training, and advice. Authors also thank the New Jersey Agricultural Experiment Station for partial financial support.
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/5
Y1 - 2021/5
N2 - Plasma-activated water (PAW) was explored as a clean-in-place agent for inactivating biofilms of Enterobacter aerogenes on the inner surface of a model piping system. Abilities of PAW or chlorine solution or distilled water to inactivate the biofilm (~1 × 108 CFU/cm2) attached to the pipe inner surface were compared. Distilled water flowing at 0.11 m/s through the piping system reduced the Enterobacter aerogenes counts on the surface by 0.35, 0.2, and 0.2 log CFU/cm2 on the tees, elbows, and tubing, respectively. However, PAW reduced the E. aerogenes counts on tees, elbows, and tubing by 3.0, 3.2, and 3.8 log CFU/cm2, respectively, which were similar to 100-ppm chlorine water. Increasing the flow velocity to 0.22 m/s made only small difference in terms of biofilm removal. The antimicrobial effect of PAW for Enterobacter aerogenes biofilm on the inner surface of a piping system was comparable to 100-ppm of chlorine solution.
AB - Plasma-activated water (PAW) was explored as a clean-in-place agent for inactivating biofilms of Enterobacter aerogenes on the inner surface of a model piping system. Abilities of PAW or chlorine solution or distilled water to inactivate the biofilm (~1 × 108 CFU/cm2) attached to the pipe inner surface were compared. Distilled water flowing at 0.11 m/s through the piping system reduced the Enterobacter aerogenes counts on the surface by 0.35, 0.2, and 0.2 log CFU/cm2 on the tees, elbows, and tubing, respectively. However, PAW reduced the E. aerogenes counts on tees, elbows, and tubing by 3.0, 3.2, and 3.8 log CFU/cm2, respectively, which were similar to 100-ppm chlorine water. Increasing the flow velocity to 0.22 m/s made only small difference in terms of biofilm removal. The antimicrobial effect of PAW for Enterobacter aerogenes biofilm on the inner surface of a piping system was comparable to 100-ppm of chlorine solution.
KW - Biofilm
KW - Clean-in-place
KW - Piping system
KW - Plasma-activated water
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U2 - 10.1016/j.ifset.2021.102664
DO - 10.1016/j.ifset.2021.102664
M3 - Article
AN - SCOPUS:85102894290
SN - 1466-8564
VL - 69
JO - Innovative Food Science and Emerging Technologies
JF - Innovative Food Science and Emerging Technologies
M1 - 102664
ER -