@article{ecc6451be4aa4e178814af77cdd5e5df,
title = "Inactivation Kinetics of G-Quadruplex/Hemin Complex and Optimization for More Reliable Catalysis",
abstract = "Reliable catalysis is critical for the synthesis of various chemicals, molecular sensing and biomedicine. G-quadruplex/Hemin (GQH) complex, a peroxidase-mimicking DNAzyme, has been widely used in various publications. However, a concern exists about the unstable kinetics of GQH-catalyzed peroxidation. This work investigates several factors that result in the inactivation of GQH and the signal degradation during long reaction periods, including pH, buffer component, the selection of substrate and the oxidation damage of cofactor. Using colorimetric and fluorescent assays, GQH was found to be highly unstable under basic conditions with 50 % of GQH activity lost within 2 minutes at high H2O2 concentrations. Appropriate conditions and substrates are suggested for accurately characterizing GQH-catalyzed reactions, as well as optimization to improve the catalytic reliability, such as the use of polyhistidine and cascade reactions. These results could be useful for GQH-related applications.",
keywords = "DNA nanostructure, DNAzyme, G-quadruplex/hemin, inactivation kinetics, peroxidation",
author = "{Monte Carlo}, {Anthony R.} and Jinglin Fu",
note = "Funding Information: This work was supported by a PECASE award to J.F. (W911NF1910240) and a DoD DURIP (W911NF2010107). J.F. is also supported by the TechAdvance Award and NJ ACTS pilot award. A.M.C. is sponsored by Rutgers-NIH MARC fellowship program and NASA New Jersey Space Grant Consortium. The authors are also grateful to the Equipment Leasing Funds from the State of New Jersey. Authors would like to thank Ezry Santiago-McRae and Sung Won Oh for training of instrument use and lab assistance. Authors also appreciated Prof Kwangwon Lee and Prof Nathan Fried for the administrative support of MARC program. A.M.C. is grateful to the Rutgers University-Camden Dean's Office for undergraduate travel funding to present this work at conferences. Funding Information: This work was supported by a PECASE award to J.F. (W911NF1910240) and a DoD DURIP (W911NF2010107). J.F. is also supported by the TechAdvance Award and NJ ACTS pilot award. A.M.C. is sponsored by Rutgers‐NIH MARC fellowship program and NASA New Jersey Space Grant Consortium. The authors are also grateful to the Equipment Leasing Funds from the State of New Jersey. Authors would like to thank Ezry Santiago‐McRae and Sung Won Oh for training of instrument use and lab assistance. Authors also appreciated Prof Kwangwon Lee and Prof Nathan Fried for the administrative support of MARC program. A.M.C. is grateful to the Rutgers University‐Camden Dean's Office for undergraduate travel funding to present this work at conferences. Publisher Copyright: {\textcopyright} 2022 The Authors. ChemPlusChem published by Wiley-VCH GmbH.",
year = "2022",
month = jul,
doi = "10.1002/cplu.202200090",
language = "English (US)",
volume = "87",
journal = "ChemPlusChem",
issn = "2192-6506",
publisher = "Wiley-VCH Verlag",
number = "7",
}