Increased levels of surgical adhesions in TGFβ1 heterozygous mice

Tyrone J. Krause, Dan Katz, Cathrine J. Wheeler, Sylvie Ebner, Randall D. McKinnon

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Adhesion formation and fibrosis represent a major complication of surgical intervention. Reducing the morbidity associated with adhesions requires an understanding of the mechanisms underlying their formation. Since increased levels of transforming growth factor-β1 (TGFβ1) have been associated with inflammation and adhesion production, we investigated the requirement of TGFβ1 in peritoneal adhesion formation utilizing mice carrying a targeted disruption of the TGFβ1 allele. Mice that were either wild-type (+/+), containing two normal alleles of TGFβ1, or heterozygous (+/-) for the TGFβ1 null allele received injections of magnesium silicate (talc), and the extent of abdominal adhesions was determined utilizing a standard grading score. Wild-type (+/+) animals had at least twofold more TGFβ1 protein in peritoneal fluids at 2 h posttrauma compared to heterozygous (+/-) mice (727 vs. 243 pg TGFβ1/mg protein by enzyme-linked immunosorbent assay (ELISA) in +/+ and +/- mice, respectively), and had significantly less scar and adhesion formation (p < .05) at 7 days posttrauma(1.8 ± 0.8 vs. 3.4 ± 1.4, graded from 0 to 5, in +/+ and +/- mice, respectively). These results demonstrate that haploid insufficiency in TGFβ1 levels can lead to inappropriate matrix and adhesion production during inflammation, and together with previous studies suggest that any perturbation of normal TGFβ1 levels can modulate the injury response that regulates the extent of adhesion formation.

Original languageEnglish (US)
Pages (from-to)31-38
Number of pages8
JournalJournal of Investigative Surgery
Volume12
Issue number1
DOIs
StatePublished - 1999

All Science Journal Classification (ASJC) codes

  • Surgery

Keywords

  • ELISA analysis
  • Surgical adhesions
  • Transforming growth factor-β1
  • Transgenic mice

Fingerprint

Dive into the research topics of 'Increased levels of surgical adhesions in TGFβ1 heterozygous mice'. Together they form a unique fingerprint.

Cite this