Induction of gp130 and LIF by differentiation inducers in human myeloid leukemia K562 cells

Ping Xie, Frances S.L. Chan, Nancy Y. Ip, Mun Fai Leung

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

It has been previously shown that phorbol 12-myristate 13-acetate (PMA), a potent differentiation inducer, induced the expression of both interleukin-6 (IL-6) and IL-6 receptor α component (IL-6Rα) in K562 leukemia cells. In the present study, we examined the ability of several differentiation inducers to regulate the expression of the signal-transducing receptor component for IL-6, gp130, and cytokine leukemia inhibitory factor (LIF) in K562 cells. We found that the expression of gp130 was dramatically induced at both the mRNA and protein levels by the two megakaryocytic inducers sodium butyrate (NaBut) and PMA. In contrast, the mRNA expression of LIF was induced by the two erythroid inducers 1-β-D-arabinofuranosyl cytosine (Ara-C) and hemin. Furthermore, activation of the PMA-induced gp130 receptor by exogenous IL-6 potentiated the differentiating effects of PMA. Our findings suggest that IL-6/gp130 signaling may be involved in the regulation of the megakaryocytic differentiation of K562 cells. Copyright (C) 1999 Elsevier Science Ltd.

Original languageEnglish (US)
Pages (from-to)1113-1119
Number of pages7
JournalLeukemia Research
Volume23
Issue number12
DOIs
StatePublished - Dec 1999
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

Keywords

  • IL-6
  • K562
  • LIF
  • Leukemia differentiation
  • PMA
  • Sodium butyrate
  • gp130

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