Inferring selective constraint from population genomic data suggests recent regulatory turnover in the human brain

Daniel R. Schrider, Andrew Kern

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The comparative genomics revolution of the past decade has enabled the discovery of functional elements in the human genomevia sequence comparison. While that is so, an important class of elements, those specific to humans, is entirelymissed by searching for sequence conservation across species. Here we present an analysis based on variation data among human genomes that utilizes a supervised machine learning approach for the identification of human-specific purifying selection in the genome. Using only allele frequency information from the complete low-coverage 1000 Genomes Project data set in conjunction with a support vector machine trained from known functional and nonfunctional portions of the genome, we are able to accurately identify portions of the genome constrained by purifying selection. Our method identifies previously known human-specific gains or losses of function and uncovers many novel candidates. Candidate targets for gain and loss of function along the human lineage include numerous putative regulatory regions of genes essential for normal development of the central nervous system, including a significant enrichment of gain of function events near neurotransmitter receptor genes. These results are consistent with regulatory turnover being a key mechanism in the evolution of human-specific characteristics of brain development. Finally, we show that the majority of the genome is unconstrained by natural selection currently, in agreement with what has been estimated from phylogenetic methods but in sharp contrast to estimates based on transcriptomics or other high-throughput functional methods.

Original languageEnglish (US)
Pages (from-to)3511-3528
Number of pages18
JournalGenome biology and evolution
Volume7
Issue number12
DOIs
StatePublished - Jan 1 2015

Fingerprint

Metagenomics
brain
genomics
turnover
genome
Genome
Brain
Forensic Anthropology
Neurotransmitter Receptor
Genetic Selection
Nucleic Acid Regulatory Sequences
Human Genome
Regulator Genes
Genomics
species conservation
Gene Frequency
gene
nervous system
neurodevelopment
natural selection

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Keywords

  • Human evolution
  • Natural selection
  • Population genetics

Cite this

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Inferring selective constraint from population genomic data suggests recent regulatory turnover in the human brain. / Schrider, Daniel R.; Kern, Andrew.

In: Genome biology and evolution, Vol. 7, No. 12, 01.01.2015, p. 3511-3528.

Research output: Contribution to journalArticle

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