Background: 2-Hydroxy-3-oxoadipate synthase is predicted to be essential for growth of Mycobacterium tuberculosis and is subject to allosteric regulation. Results: The role of regulators was characterized by kinetics and detection of thiamin diphosphate-bound covalent intermediate distribution. Conclusion: AcCoA activates steps leading to a predecarboxylation intermediate; GarA chiefly inhibits postdecarboxylation steps. Significance: This novel regulatory regime in thiamin diphosphate enzymes provides new leads to inhibition.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology