Influence of insulin therapy on burn wound healing in rats

Sundararajan V. Madibally, Vered Solomon, Richard N. Mitchell, Livingston Van De Water, Martin L. Yarmush, Mehmet Toner

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


Background. Insulin is proposed as a therapy for suppressing muscle wasting after burn trauma although the long-term effects of this therapy on wound healing are not yet known. The present study was designed to investigate the effect of systemically administered insulin therapy on burn wound healing. Materials and methods. Young rats weighing 80-150 g were subjected to 15-20% total body surface area burn injury on their shaved dorsum. The insulin dosage was increased over the first 3 days in each rat from 0.25 U (Day 1), 0.5 U (Day 2), and 1.0 U (Day 3) per 100 g body wt. The rats were euthanized at the fourth or fifteenth day postinjury. Skin sections were analyzed by histochemistry and quantitative polarization microscopy. Results. Histology showed a decreased number of inflammatory cells and increased vasodilation in the insulin-treated animals at Day 4 relative to untreated rats; at Day 15 there was increased reepithelialization. Quantitative analysis using polarization microscopy and picrosirius red staining showed an increased collagen deposition in wounds by Day 4 in insulin-treated rats relative to untreated burn controls. Conclusion. These results indicate that insulin induces accelerated wound healing associated with diminished inflammation and increased collagen deposition.

Original languageEnglish (US)
Pages (from-to)92-100
Number of pages9
JournalJournal of Surgical Research
Issue number2
StatePublished - Feb 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Surgery


  • Burn injury
  • Insulin
  • Muscle wasting
  • Polarization microscopy
  • Wound healing


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