TY - JOUR
T1 - Influence of physico-chemical changes on enzymatic digestibility of ionic liquid and AFEX pretreated corn stover
AU - Li, Chenlin
AU - Cheng, Gang
AU - Balan, Venkatesh
AU - Kent, Michael S.
AU - Ong, Markus
AU - Chundawat, Shishir P.S.
AU - Sousa, Leonardo da Costa
AU - Melnichenko, Yuri B.
AU - Dale, Bruce E.
AU - Simmons, Blake A.
AU - Singh, Seema
N1 - Funding Information:
The authors thank Novozymes for the gift of the enzyme cocktails used in this work. This work was part of the DOE Joint BioEnergy Institute ( http://www.jbei.org ) supported by the US Department of Energy, Office of Science, Office of Biological and Environmental Research , through contract DE-AC02-05CH11231 between Lawrence Berkeley National Laboratory and the US Department of Energy. This work was also supported in part by US Department of Energy through the DOE Great Lakes Bioenergy Research Center (GLBRC) Grant DE-FC02-07ER64494 . The research conducted at Oak Ridge National Laboratory’s High Flux Isotope Reactor was sponsored by the Scientific User Facilities Division, Office of Basic Energy Sciences, US Department of Energy. G. Cheng acknowledges the EPSCoR Neutron Travel Fellowship through University of Tennessee. We would like to thank Derek Marshall for helping prepare AFEX pretreated biomass.
PY - 2011/7
Y1 - 2011/7
N2 - Ionic liquid (IL) and ammonia fiber expansion (AFEX) pretreatments were studied to develop the first direct side-by-side comparative assessment on their respective impacts on biomass structure, composition, process mass balance, and enzymatic saccharification efficiency. AFEX pretreatment completely preserves plant carbohydrates, whereas IL pretreatment extracts 76% of hemicellulose. In contrast to AFEX, the native crystal structure of the recovered corn stover from IL pretreatment was significantly disrupted. For both techniques, more than 70% of the theoretical sugar yield was attained after 48 h of hydrolysis using commercial enzyme cocktails. IL pretreatment requires less enzyme loading and a shorter hydrolysis time to reach 90% yields. Hemicellulase addition led to significant improvements in the yields of glucose and xylose for AFEX pretreated corn stover, but not for IL pretreated stover. These results provide new insights into the mechanisms of IL and AFEX pretreatment, as well as the advantages and disadvantages of each.
AB - Ionic liquid (IL) and ammonia fiber expansion (AFEX) pretreatments were studied to develop the first direct side-by-side comparative assessment on their respective impacts on biomass structure, composition, process mass balance, and enzymatic saccharification efficiency. AFEX pretreatment completely preserves plant carbohydrates, whereas IL pretreatment extracts 76% of hemicellulose. In contrast to AFEX, the native crystal structure of the recovered corn stover from IL pretreatment was significantly disrupted. For both techniques, more than 70% of the theoretical sugar yield was attained after 48 h of hydrolysis using commercial enzyme cocktails. IL pretreatment requires less enzyme loading and a shorter hydrolysis time to reach 90% yields. Hemicellulase addition led to significant improvements in the yields of glucose and xylose for AFEX pretreated corn stover, but not for IL pretreated stover. These results provide new insights into the mechanisms of IL and AFEX pretreatment, as well as the advantages and disadvantages of each.
KW - AFEX
KW - Crystallinity
KW - Enzymatic saccharification
KW - Ionic liquid
KW - Surface area
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U2 - 10.1016/j.biortech.2011.04.005
DO - 10.1016/j.biortech.2011.04.005
M3 - Article
C2 - 21531133
AN - SCOPUS:79956347286
SN - 0960-8524
VL - 102
SP - 6928
EP - 6936
JO - Bioresource Technology
JF - Bioresource Technology
IS - 13
ER -