TY - JOUR
T1 - Infrared reflection-absorption spectroscopy
T2 - Principles and applications to lipid-protein interaction in Langmuir films
AU - Mendelsohn, Richard
AU - Mao, Guangru
AU - Flach, Carol R.
N1 - Funding Information:
Some experiments conducted at Rutgers University reported in this article were supported by NIH AI-83222 . We also thank the authors of various figures (as noted) for their kindness in allowing us to reprint their work and in many cases supplying high-quality figures.
PY - 2010/4
Y1 - 2010/4
N2 - Infrared reflection-absorption spectroscopy (IRRAS) of lipid/protein monolayer films in situ at the air/water interface provides unique molecular structure and orientation information from the film constituents. The technique is thus well suited for studies of lipid/protein interaction in a physiologically relevant environment. Initially, the nature of the IRRAS experiment is described and the molecular structure information that may be obtained is recapitulated. Subsequently, several types of applications, including the determination of lipid chain conformation and tilt as well as elucidation of protein secondary structure are reviewed. The current article attempts to provide the reader with an understanding of the current capabilities of IRRAS instrumentation and the type of results that have been achieved to date from IRRAS studies of lipids, proteins, and lipid/protein films of progressively increasing complexity. Finally, possible extensions of the technology are briefly considered.
AB - Infrared reflection-absorption spectroscopy (IRRAS) of lipid/protein monolayer films in situ at the air/water interface provides unique molecular structure and orientation information from the film constituents. The technique is thus well suited for studies of lipid/protein interaction in a physiologically relevant environment. Initially, the nature of the IRRAS experiment is described and the molecular structure information that may be obtained is recapitulated. Subsequently, several types of applications, including the determination of lipid chain conformation and tilt as well as elucidation of protein secondary structure are reviewed. The current article attempts to provide the reader with an understanding of the current capabilities of IRRAS instrumentation and the type of results that have been achieved to date from IRRAS studies of lipids, proteins, and lipid/protein films of progressively increasing complexity. Finally, possible extensions of the technology are briefly considered.
KW - Aqueous monolayers
KW - Infrared spectroscopy
KW - Langmuir films
KW - Lipid conformation
KW - Protein secondary structure
KW - Pulmonary surfactant
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U2 - 10.1016/j.bbamem.2009.11.024
DO - 10.1016/j.bbamem.2009.11.024
M3 - Review article
C2 - 20004639
AN - SCOPUS:77449130066
SN - 0005-2736
VL - 1798
SP - 788
EP - 800
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 4
ER -