Inhibition by CIPC of mitosis and development in Dictyostelium discoideum and the isolation of CIPC‐resistant mutants

Eileen White, Dorothea Scandella, Eugene R. Katz

Research output: Contribution to journalArticle

3 Scopus citations


The anti‐mitotic herbicide isopropyl N‐(3‐chlorophenyl) carbamate (CIPC) prevents the growth of amoebae of Dictyostelium discoideum without killing the cells for a period of time equivalent to one generation. During in‐hibition, amoebae accumulate in prophase and metaphase of mitosis. After removal of CIPC, they continue through mitosis and then divide. The addition of CIPC to amoebae under starvation conditions prevents aggregation and concomitant cell elongation. The cells, however, do not lose their ability to adhere to a surface, and they remain viable. When CIPC is added to amoebae which have formed streams, it leads to the disintegration of streams into small clusters of cells and to a loss of cell elongation. Post‐aggregation stages of development can be inhibited by CIPC at the mound, slug, or Mexican hat stages. Slugs break apart into distinct aggregates. Mutants resistant to CIPC can be obtained easily. Among these mutants, many become temperature sensititive for growth (27°C) or development (27°C or 15.5°C). Others show various abnormalities at the normal temperature (22°C). Most mutants are cross resistant to the microtubule inhibitors nocodazole and thiabendazole, and some are also resistant to CIPC during development.

Original languageEnglish (US)
Pages (from-to)99-111
Number of pages13
JournalDevelopmental Genetics
Issue number1
StatePublished - Jan 1 1981
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology
  • Cell Biology


  • CIPC as a mitotic inhibitor of Dictyostelium
  • CIPC‐resistant mutants
  • Dictyostelium
  • inhibition of Dictyostelium development by CIPC

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