Inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced inflammatory skin edema and ornithine decarboxylase activity by theaflavin-3,3’-digallate in mouse

Yu Chih Liang, De Cheng Tsai, Shoei Yn Lin-Shiau, Chieh Fu Chen, Chi Tang Ho, Jen Kun Lin

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Among black tea polyphenols, theaflavins were generally considered to be the most effective in cancer chemoprevention. In this study, we examined the inhibitory effects of black tea polyphenols, including theaflavin (TF-1), a mixture (TF-2) of theaflavin-3-gallate and theaflavin-3′-gallate, theaflavin-3,3′-digallate (TF-3), and the green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced edema and ornithine decarboxylase (ODC) activity. Topical application of these polyphenols onto the mouse resulted in inhibition of TPA-induced ear edema and skin epidermal ODC activity. The inhibitory order was as follows: TF-3 > TF-2≅EGCG > TF-1. Western and Northern blots indicated that TF-3 significantly reduced the protein and mRNA levels of ODC in TPA-treated mouse skin and NIH 3T3 cells, whereas EGCG showed less activity. EGCG and TF-3 were able to inhibit the ODC enzyme activity in vitro. Furthermore, TF-3 also significantly reduced the basal promoter activity of the ODC gene in NIH 3T3 cells that were transiently transfected with ODC reporter plasmid. These results suggested that TF-3 was a potential inhibitor of ODC activity and TPA-induced edema and might be effective in cancer chemoprevention.

Original languageEnglish (US)
Pages (from-to)217-223
Number of pages7
JournalNutrition and Cancer
Volume42
Issue number2
DOIs
StatePublished - Jan 1 2002

Fingerprint

Ornithine Decarboxylase
Tetradecanoylphorbol Acetate
Edema
Skin
Polyphenols
Tea
NIH 3T3 Cells
Chemoprevention
theaflavin
Northern Blotting
Ear
Neoplasms
Plasmids
Western Blotting
Messenger RNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Oncology
  • Nutrition and Dietetics
  • Cancer Research

Cite this

Liang, Yu Chih ; Tsai, De Cheng ; Lin-Shiau, Shoei Yn ; Chen, Chieh Fu ; Ho, Chi Tang ; Lin, Jen Kun. / Inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced inflammatory skin edema and ornithine decarboxylase activity by theaflavin-3,3’-digallate in mouse. In: Nutrition and Cancer. 2002 ; Vol. 42, No. 2. pp. 217-223.
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abstract = "Among black tea polyphenols, theaflavins were generally considered to be the most effective in cancer chemoprevention. In this study, we examined the inhibitory effects of black tea polyphenols, including theaflavin (TF-1), a mixture (TF-2) of theaflavin-3-gallate and theaflavin-3′-gallate, theaflavin-3,3′-digallate (TF-3), and the green tea polyphenol (-)-epigallocatechin-3-gallate (EGCG) on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced edema and ornithine decarboxylase (ODC) activity. Topical application of these polyphenols onto the mouse resulted in inhibition of TPA-induced ear edema and skin epidermal ODC activity. The inhibitory order was as follows: TF-3 > TF-2≅EGCG > TF-1. Western and Northern blots indicated that TF-3 significantly reduced the protein and mRNA levels of ODC in TPA-treated mouse skin and NIH 3T3 cells, whereas EGCG showed less activity. EGCG and TF-3 were able to inhibit the ODC enzyme activity in vitro. Furthermore, TF-3 also significantly reduced the basal promoter activity of the ODC gene in NIH 3T3 cells that were transiently transfected with ODC reporter plasmid. These results suggested that TF-3 was a potential inhibitor of ODC activity and TPA-induced edema and might be effective in cancer chemoprevention.",
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Inhibition of 12-O-tetradecanoylphorbol-13-acetate-induced inflammatory skin edema and ornithine decarboxylase activity by theaflavin-3,3’-digallate in mouse. / Liang, Yu Chih; Tsai, De Cheng; Lin-Shiau, Shoei Yn; Chen, Chieh Fu; Ho, Chi Tang; Lin, Jen Kun.

In: Nutrition and Cancer, Vol. 42, No. 2, 01.01.2002, p. 217-223.

Research output: Contribution to journalArticle

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AU - Liang, Yu Chih

AU - Tsai, De Cheng

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AU - Ho, Chi Tang

AU - Lin, Jen Kun

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