Inhibition of gastric mucosal laminin receptor by Helicobacter pylori lipopolysaccharide

B. L. Slomiany, J. Piotrowski, S. Sengupta, A. Slomiany

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Laminin receptor was isolated from gastric epithelial cell membrane by the procedure involving membrane solubilization with octylglucoside followed by affinity chromatography on laminin-coupled Sepharose. The receptor protein, eluted from the matrix with cation-free EDTA buffer, yielded on SDS-PAGE a single 67kDa band. After radioiodination, the protein was incorporated into liposomes which displayed specific affinity toward the laminin-coated surface. The binding of liposomal receptor to the laminin-coated surface was inhibited by lipopolysaccharide from H.pylori. The inhibitory effect was proportional to the concentration of lipopolysaccharide up to 50μg/ml at which point a 96% decrease in the receptor binding occurred. It is suggested that a similar process may account for the loss of mucosal integrity in the pathogenesis of H. pylori associated gastric disease.

Original languageEnglish (US)
Pages (from-to)963-970
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Mar 29 1991

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Inhibition of gastric mucosal laminin receptor by Helicobacter pylori lipopolysaccharide'. Together they form a unique fingerprint.

Cite this