Inhibition of the cardiac protein kinase A-dependent chloride conductance by endothelin-1

Andrew F. James, Lai Hua Xie, Yasushi Fujitani, Seiji Hayashi, Minoru Horie

Research output: Contribution to journalArticlepeer-review

81 Scopus citations

Abstract

Endothelin-1 is a peptide hormone constitutively secreted by vascular and endocardial endothelial cells1-3. Secretion of endothelin-1 is increased under certain pathophysiological conditions, including coronary vasospasm, cardiac ischaemia and myocardial infarction. We have examined the effect of endothelin-1 on the protein kinase A (PKA)-dependent chloride current in voltage-clamped guinea pig ventricular myocytes. This conductance, induced by catecholamines through β-adrenergic receptors, counteracts the simultaneously increased L-type calcium current by shortening the action potential duration4-6. We report here that endothelin-1, acting through ETA (endothelin-1-selective) receptors, inhibited the current through a pertussis toxin-sensitive mechanism, analogous to muscarinic receptors, by reducing the intracellular cyclic AMP concentration. This effect of endothelin-1 should help protect the ventricle against potentially arrhythmogenic shortening of the action potential during ischaemia when the circulating levels of catecholamines are increased.

Original languageEnglish (US)
Pages (from-to)297-300
Number of pages4
JournalNature
Volume370
Issue number6487
DOIs
StatePublished - 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General

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