Inhibition of xanthine oxidase and suppression of intracellular reactive oxygen species in HL-60 cells by theaflavin-3,3'-digallate, (-)- epigallocatechin-3-gallate, and propyl gallate

Jen Kun Lin, Ping Chung Chen, Chi Tang Ho, Shoei Yn Lin-Shiau

Research output: Contribution to journalArticlepeer-review

162 Scopus citations

Abstract

The inhibitory effects of five tea polyphenols, namely theaflavin (TF1), theaflavin-3-gallate (TF2), theaflavin-3,3'-digallate (TF3), (-)- epigallocatechin-3-gallate (EGCG), and gallic acid, and propyl gallate (PG) on xanthine oxidase (XO) were investigated. These six antioxidant compounds reduce oxidative stress. Theaflavins and EGCG inhibit XO to produce uric acid and also act as scanvengers of superoxide. TF3 acts as a competitive inhibitor and is the most potent inhibitor of XO among these compounds. Tea polyphenols and PG all have potent inhibitory effects (>50%) on PMA- stimulated superoxide production at 20~50 μM in HL-60 cells. Gallic acid (GA) showed no inhibition under the same conditions. At 10 μM, only EGCG, TF3, and PG showed significant inhibition with potency of PG > EGCG > TF3. The superoxide scavenging abilities of these six compounds are as follows: EGCG > TF2 > TF1 > GA > TF3 > PG. PG was the most potent inhibitor of PMA- stimulated H2O2 production in HL-60 cells. The order of H2O2 scavenging ability was TF2 > TF3 > TF1 > EGCG > PG > GA. Therefore, the antioxidative activity of tea polyphenols and PG is due not only to their ability to scavenge superoxides but also to their ability to block XO and related oxidative signal transducers.

Original languageEnglish (US)
Pages (from-to)2736-2743
Number of pages8
JournalJournal of agricultural and food chemistry
Volume48
Issue number7
DOIs
StatePublished - Jul 1 2000

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Keywords

  • Allopurinol
  • EGCG
  • Hydrogen peroxide
  • ROS
  • Superoxide
  • Theaflavin-3,3'-digallate
  • Xantine oxidase

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