Inoculum size of Clostridium botulinum 56A spores influences time-to-detection and percent growth-positive samples

L. Zhao, T. J. Montville, D. W. Schaffner

Research output: Contribution to journalArticlepeer-review

38 Scopus citations

Abstract

The influence of inoculum size on the growth kinetics of Clostridium botulinum 56A and percentage of growth-positive samples was studied in a complete factorial design with factors of inoculum size (1, 100, or 10,000 spores), pH, and sodium-chloride concentration. Growth was followed hourly as change in A620. Polynomial regression was used to analyze the data. The time-to-detection and percent growth-positive samples were significantly affected by inoculum size and its quadratic term. When inoculum size increased from 1 to 100 spores/sample, the percent growth-positive samples increased, and the time-to-detection decreased. When the inoculum was 1000 spores/sample or higher, there was little additional effect. Inoculum size might influence results through simple probability or quorum sensing. The maximum growth rate was independent of inoculum levels.

Original languageEnglish (US)
Pages (from-to)1369-1375
Number of pages7
JournalJournal of Food Science
Volume65
Issue number8
DOIs
StatePublished - 2000

All Science Journal Classification (ASJC) codes

  • Food Science

Keywords

  • C. botulinum
  • Gompertz model
  • Inoculum size
  • Quorum sensing
  • Spores

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