Insulin stimulates monocyte membrane associated tissue factor activity

B. A. Bhatt, D. R. Patel, D. Kristol, R. R. Arora, Charles Spillert

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The benefits of intensive insulin therapy for type 2 diabetics with cardiovascular disease (CVD) are highly controversial. We performed in vitro studies to determine whether insulin has any effect on whole blood coagulation and assess how insulin responds to monocyte membrane associated tissue factor (mmaTF) induced hypercoagulability. Mercuric ion (Hg2+), which has been shown to increase mmaTF-dependent procoagulant activity in whole blood, was used to stimulate similar pathways in our experiment. We spiked human citrated whole blood with HgCl2 and Humulin-N® (HN) at final concentrations of control, 0.02 U/mL HN, 0.004 U/mL HN, 0.002 U/mL HN, 0.005% HgCl2, 0.02 U/mL HN:0.005% HgCl2, 0.004 U/mL HN:0.005% HgCl2, and 0.002 U/mL HN:0.005% HgCl2. The samples (n=12) were incubated at 37°C for 10 minutes and recalcified with CaCl2 to initiate clotting. The clotting time (CT) was measured with an Amelung KC4A Micro. The results indicate that HN by itself has no effect on CT. However, each concentration of HN:HgCl2 exhibited a shorter CT than either HN or HgCl2 alone (p<0.02 in each case). Studies with bovine pancreas insulin yielded similar results. This data indicates that insulin is stimulating mmaTF activity, and warrants the implementation of coagulation monitoring protocols for intensive insulin therapy.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE 29th Annual Northeast Bioengineering Conference
EditorsStanley Reisman, Richard Foulds, Bruno Mantilla
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages251-252
Number of pages2
ISBN (Electronic)0780377672
DOIs
StatePublished - Jan 1 2003
Event29th IEEE Annual Northeast Bioengineering Conference, NEBC 2003 - Newark, United States
Duration: Mar 22 2003Mar 23 2003

Publication series

NameProceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC
Volume2003-January
ISSN (Print)1071-121X
ISSN (Electronic)2160-7001

Other

Other29th IEEE Annual Northeast Bioengineering Conference, NEBC 2003
CountryUnited States
CityNewark
Period3/22/033/23/03

All Science Journal Classification (ASJC) codes

  • Bioengineering

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  • Cite this

    Bhatt, B. A., Patel, D. R., Kristol, D., Arora, R. R., & Spillert, C. (2003). Insulin stimulates monocyte membrane associated tissue factor activity. In S. Reisman, R. Foulds, & B. Mantilla (Eds.), Proceedings of the IEEE 29th Annual Northeast Bioengineering Conference (pp. 251-252). [1216089] (Proceedings of the IEEE Annual Northeast Bioengineering Conference, NEBEC; Vol. 2003-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEBC.2003.1216089